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Bioengineering for robust tolerance against cold and drought stresses via co-overexpressing three Cu-miRNAs in major food crops.
- Source :
-
Cell reports [Cell Rep] 2024 Oct 22; Vol. 43 (10), pp. 114828. Date of Electronic Publication: 2024 Oct 04. - Publication Year :
- 2024
-
Abstract
- Environmental stresses threaten global food security by reducing major crop productivity. MicroRNAs (miRNAs), a class of small non-coding RNAs, function as master regulators of gene expression in plants. In this study, we co-overexpressed three copper-miRNAs (miR397, miR408, and miR528) in three major food crops (referred to as 3miR-OE), which simultaneously silenced several target laccase genes, resulting in reduced lignin contents but increased flavonoid metabolites. Importantly, we observed that, compared to wild-type and single miRNA overexpression lines, the 3miR-OE transgenic Japonica and Indica rice exhibited significantly enhanced tolerance against cold and drought stresses throughout the growth period. In addition, 3miR-OE transgenic maize and wheat also exhibited robust resistance to cold and water-deficit conditions, suggesting that co-overexpressing three Cu-miRNAs is a powerful tool for improving resilience to abiotic stresses across diverse crops. Altogether, we have developed a bioengineering strategy to maintain crop growth and yield under unfavorable environmental conditions.<br />Competing Interests: Declaration of interests The authors declare no competing interests.<br /> (Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.)
- Subjects :
- Bioengineering methods
Oryza genetics
Oryza growth & development
Cold Temperature
Zea mays genetics
Zea mays metabolism
Laccase genetics
Laccase metabolism
Adaptation, Physiological genetics
Droughts
Crops, Agricultural genetics
Plants, Genetically Modified
MicroRNAs genetics
MicroRNAs metabolism
Stress, Physiological genetics
Gene Expression Regulation, Plant
Copper metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2211-1247
- Volume :
- 43
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Cell reports
- Publication Type :
- Academic Journal
- Accession number :
- 39368086
- Full Text :
- https://doi.org/10.1016/j.celrep.2024.114828