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Structural characterisation of the complete cycle of sliding clamp loading in Escherichia coli.
- Source :
-
Nature communications [Nat Commun] 2024 Sep 27; Vol. 15 (1), pp. 8372. Date of Electronic Publication: 2024 Sep 27. - Publication Year :
- 2024
-
Abstract
- Ring-shaped DNA sliding clamps are essential for DNA replication and genome maintenance. Clamps need to be opened and chaperoned onto DNA by clamp loader complexes (CLCs). Detailed understanding of the mechanisms by which CLCs open and place clamps around DNA remains incomplete. Here, we present a series of six structures of the Escherichia coli CLC bound to an open or closed clamp prior to and after binding to a primer-template DNA, representing the most significant intermediates in the clamp loading process. We show that the ATP-bound CLC first binds to a clamp, then constricts to hold onto it. The CLC then expands to open the clamp with a gap large enough for double-stranded DNA to enter. Upon binding to DNA, the CLC constricts slightly, allowing clamp closing around DNA. These structures provide critical high-resolution snapshots of clamp loading by the E. coli CLC, revealing how the molecular machine works.<br /> (© 2024. The Author(s).)
- Subjects :
- Adenosine Triphosphate metabolism
DNA Replication
Models, Molecular
Cryoelectron Microscopy
DNA Polymerase III metabolism
DNA Polymerase III chemistry
Escherichia coli metabolism
Escherichia coli genetics
Escherichia coli Proteins metabolism
Escherichia coli Proteins chemistry
Escherichia coli Proteins genetics
DNA, Bacterial metabolism
DNA, Bacterial genetics
Subjects
Details
- Language :
- English
- ISSN :
- 2041-1723
- Volume :
- 15
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Nature communications
- Publication Type :
- Academic Journal
- Accession number :
- 39333521
- Full Text :
- https://doi.org/10.1038/s41467-024-52623-9