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The Arabidopsis splicing factor PORCUPINE/SmE1 orchestrates temperature-dependent root development via auxin homeostasis maintenance.
- Source :
-
The New phytologist [New Phytol] 2024 Nov; Vol. 244 (4), pp. 1408-1421. Date of Electronic Publication: 2024 Sep 27. - Publication Year :
- 2024
-
Abstract
- Appropriate abiotic stress response is pivotal for plant survival and makes use of multiple signaling molecules and phytohormones to achieve specific and fast molecular adjustments. A multitude of studies has highlighted the role of alternative splicing in response to abiotic stress, including temperature, emphasizing the role of transcriptional regulation for stress response. Here we investigated the role of the core-splicing factor PORCUPINE (PCP) on temperature-dependent root development. We used marker lines and transcriptomic analyses to study the expression profiles of meristematic regulators and mitotic markers, and chemical treatments, as well as root hormone profiling to assess the effect of auxin signaling. The loss of PCP significantly alters RAM architecture in a temperature-dependent manner. Our results indicate that PCP modulates the expression of central meristematic regulators and is required to maintain appropriate levels of auxin in the RAM. We conclude that alternative pre-mRNA splicing is sensitive to moderate temperature fluctuations and contributes to root meristem maintenance, possibly through the regulation of phytohormone homeostasis and meristematic activity.<br /> (© 2024 The Author(s). New Phytologist © 2024 New Phytologist Foundation.)
- Subjects :
- Gene Expression Regulation, Plant drug effects
Homeostasis
Plant Roots growth & development
Plant Roots metabolism
Plant Roots genetics
Temperature
Arabidopsis genetics
Arabidopsis growth & development
Arabidopsis metabolism
Arabidopsis Proteins metabolism
Arabidopsis Proteins genetics
Indoleacetic Acids metabolism
Meristem growth & development
Meristem metabolism
Meristem genetics
RNA Splicing Factors metabolism
RNA Splicing Factors genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1469-8137
- Volume :
- 244
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- The New phytologist
- Publication Type :
- Academic Journal
- Accession number :
- 39327913
- Full Text :
- https://doi.org/10.1111/nph.20153