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Association of Microbleeds on Susceptibility-Weighted Imaging with Ferroptosis and Prognosis in Rabbits with Spinal Cord Injury.
- Source :
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Discovery medicine [Discov Med] 2024 Sep; Vol. 36 (188), pp. 1869-1879. - Publication Year :
- 2024
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Abstract
- Background: Susceptibility-weighted imaging (SWI) is a common imaging technique used to identify cerebral microbleeds. Given that spinal cord injury (SCI) often creates an environment that favors ferroptosis, a type of cell death driven by iron, this study aimed to explore the relationship between microbleeds on SWI and ferroptosis, and explore the effect of deferoxamine on SCI.<br />Methods: Thirty-six rabbits were divided into three groups: sham, SCI, and SCI with deferoxamine (DFO, a ferroptosis inhibitor) treatment (SCI+DFO). Following 48 hours of SCI modeling, the rabbits underwent magnetic resonance imaging (MRI) and SWI examinations. Ferroptosis markers and spinal cord tissue morphology were examined, and the modified Tarlov's score was used to assess neurological function.<br />Results: SWI analysis revealed that rabbits in the SCI group exhibited lower signal intensities and larger microbleed areas compared to the those in the SCI+DFO group ( p < 0.05). The SCI+DFO group demonstrated significantly decreased iron and malondialdehyde (MDA) levels, coupled with increased glutathione (GSH) and glutathione peroxidase 4 (GPX4) levels, along with attenuated ferroptosis ( p < 0.05). This group also displayed greater Neuronal Nuclei (NeuN) expression, Tarlov's scores, and neurological recovery rates (all p < 0.05). A significant positive correlation was found between the microbleed area and iron content (r = 0.59, p = 0.04), MDA (r = 0.75, p = 0.01), and mitochondrial damage (r = 0.90, p < 0.01). Conversely, a negative correlation was established between the microbleed area and GPX4 levels (r = -0.87, p < 0.01), as well as neurological function recovery (r = -0.62, p = 0.03).<br />Conclusion: The extent of microbleeds on SWI following SCI is closely correlated with ferroptosis, and the inhibition of ferroptosis could improve neurologic function. These findings suggest that the area of microbleeds on SWI could potentially serve as a predictive marker for ferroptosis in spinal cord injury.
- Subjects :
- Animals
Rabbits
Iron metabolism
Prognosis
Male
Disease Models, Animal
Spinal Cord diagnostic imaging
Spinal Cord pathology
Spinal Cord metabolism
Ferroptosis drug effects
Spinal Cord Injuries complications
Spinal Cord Injuries diagnostic imaging
Spinal Cord Injuries pathology
Magnetic Resonance Imaging methods
Deferoxamine pharmacology
Deferoxamine therapeutic use
Subjects
Details
- Language :
- English
- ISSN :
- 1944-7930
- Volume :
- 36
- Issue :
- 188
- Database :
- MEDLINE
- Journal :
- Discovery medicine
- Publication Type :
- Academic Journal
- Accession number :
- 39327250
- Full Text :
- https://doi.org/10.24976/Discov.Med.202436188.173