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Cross-species single-cell spatial transcriptomic atlases of the cerebellar cortex.
- Source :
-
Science (New York, N.Y.) [Science] 2024 Sep 27; Vol. 385 (6716), pp. eado3927. Date of Electronic Publication: 2024 Sep 27. - Publication Year :
- 2024
-
Abstract
- The molecular and cellular organization of the primate cerebellum remains poorly characterized. We obtained single-cell spatial transcriptomic atlases of macaque, marmoset, and mouse cerebella and identified primate-specific cell subtypes, including Purkinje cells and molecular-layer interneurons, that show different expression of the glutamate ionotropic receptor Delta type subunit 2 ( GRID2 ) gene. Distinct gene expression profiles were found in anterior, posterior, and vestibular regions in all species, whereas region-selective gene expression was predominantly observed in the granular layer of primates and in the Purkinje layer of mice. Gene expression gradients in the cerebellar cortex matched well with functional connectivity gradients revealed with awake functional magnetic resonance imaging, with more lobule-specific differences between primates and mice than between two primate species. These comprehensive atlases and comparative analyses provide the basis for understanding cerebellar evolution and function.
- Subjects :
- Animals
Male
Mice
Interneurons metabolism
Magnetic Resonance Imaging
Purkinje Cells metabolism
Receptors, Ionotropic Glutamate genetics
Receptors, Ionotropic Glutamate metabolism
Single-Cell Analysis
Species Specificity
Atlases as Topic
Callithrix anatomy & histology
Callithrix genetics
Cerebellar Cortex metabolism
Cerebellar Cortex ultrastructure
Macaca anatomy & histology
Macaca genetics
Receptors, Glutamate metabolism
Receptors, Glutamate genetics
Transcriptome
Connectome
Subjects
Details
- Language :
- English
- ISSN :
- 1095-9203
- Volume :
- 385
- Issue :
- 6716
- Database :
- MEDLINE
- Journal :
- Science (New York, N.Y.)
- Publication Type :
- Academic Journal
- Accession number :
- 39325889
- Full Text :
- https://doi.org/10.1126/science.ado3927