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pH-Sensitive blue and red N-CDs for L-asparaginase quantification in complex biological matrices.
- Source :
-
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy [Spectrochim Acta A Mol Biomol Spectrosc] 2025 Jan 15; Vol. 325, pp. 125161. Date of Electronic Publication: 2024 Sep 19. - Publication Year :
- 2025
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Abstract
- A novel fluorometric method for the determination of L-asparaginase, an enzyme crucial in cancer therapy and food industry applications, is presented. This sensitive and selective approach utilizes L-asparagine and two pH-sensitive carbon dots (blue-N-CDs and red-N-CDs) as probes. The interaction between L-asparagine and L-asparaginase liberates ammonia, causing an increase in pH. This pH change simultaneously decreases the fluorescence of blue-N-CDs while enhancing the emission of red-N-CDs, enabling ratiometric detection of L-asparaginase. Comprehensive characterization of both carbon dots and investigation of their response mechanism towards L-asparaginase were conducted using ultraviolet-visible spectrophotometry, fluorescence spectroscopy, and transmission electron microscopy (TEM) imaging techniques. The designed approach demonstrates outstanding linearity (20 to 2000 U L <superscript>-1</superscript> ) and a low detection limit (6.95 U L <superscript>-1</superscript> ) for L-asparaginase quantification. Moreover, when tested to human serum samples, the detection system exhibits outstanding selectivity and high recovery rates (96.15% to 99.75%) with low standard deviation, underscoring its suitability for practical implementation in clinical diagnostics.<br />Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br /> (Copyright © 2024 Elsevier B.V. All rights reserved.)
- Subjects :
- Hydrogen-Ion Concentration
Humans
Limit of Detection
Quantum Dots chemistry
Cadmium Compounds chemistry
Asparagine chemistry
Asparagine metabolism
Asparagine analysis
Spectrophotometry, Ultraviolet
Sulfides chemistry
Asparaginase chemistry
Asparaginase analysis
Asparaginase metabolism
Asparaginase blood
Spectrometry, Fluorescence
Subjects
Details
- Language :
- English
- ISSN :
- 1873-3557
- Volume :
- 325
- Database :
- MEDLINE
- Journal :
- Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy
- Publication Type :
- Academic Journal
- Accession number :
- 39316858
- Full Text :
- https://doi.org/10.1016/j.saa.2024.125161