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Linoleic Acid Promotes Mitochondrial Biogenesis and Alleviates Acute Lung Injury.
- Source :
-
The clinical respiratory journal [Clin Respir J] 2024 Sep; Vol. 18 (9), pp. e70004. - Publication Year :
- 2024
-
Abstract
- Introduction: Acute lung injury (ALI) is a critical and lethal medical condition. This syndrome is characterized by an imbalance in the body's oxidation stress and inflammation. Linoleic acid (LA), a polyunsaturated fatty acid, has been extensively studied for its potential health benefits, including anti-inflammatory and antioxidant activities. However, the therapeutic effects of LA on ALI remain unexplored.<br />Methods: Lipopolysaccharide (LPS), found in gram-negative bacteria's outer membrane, was intraperitoneally injected to induce ALI in mice. In vitro model was established by LPS stimulation of mouse lung epithelial 12 (MLE-12) cells.<br />Results: LA treatment demonstrated a significant amelioration in LPS-induced hypothermia, poor state, and pulmonary injury in mice. LA treatment resulted in a reduction in the concentration of bronchoalveolar lavage fluid (BALF) protein and an increase in myeloperoxidase (MPO) activity in LPS-induced mice. LA treatment reduced the generation of white blood cells. LA treatment reduced cell-free (cfDNA) release and promote adenosine triphosphate (ATP) production. LA increased the levels of superoxide dismutase (SOD) and glutathione (GSH) but decreased the production of malondialdehyde (MDA). LA treatment enhanced mitochondrial membrane potential. LA attenuated LPS-induced elevations of inflammatory cytokines in both mice and cells. Additionally, LA exerted its protective effect against LPS-induced damage through activation of the peroxisome proliferator-activated receptor γ coactivator l alpha (PGC-1α)/nuclear respiratory factor 1 (NRF1)/transcription factor A of the mitochondrion (TFAM) pathway.<br />Conclusion: LA may reduce inflammation and stimulate mitochondrial biogenesis in ALI mice and MLE-12 cells.<br /> (© 2024 The Author(s). The Clinical Respiratory Journal published by John Wiley & Sons Ltd.)
- Subjects :
- Animals
Mice
Male
Oxidative Stress drug effects
Mitochondria metabolism
Mitochondria drug effects
Bronchoalveolar Lavage Fluid
Acute Lung Injury metabolism
Acute Lung Injury pathology
Acute Lung Injury drug therapy
Linoleic Acid metabolism
Linoleic Acid pharmacology
Organelle Biogenesis
Lipopolysaccharides
Disease Models, Animal
Subjects
Details
- Language :
- English
- ISSN :
- 1752-699X
- Volume :
- 18
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- The clinical respiratory journal
- Publication Type :
- Academic Journal
- Accession number :
- 39313818
- Full Text :
- https://doi.org/10.1111/crj.70004