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An AIE-based ratiometric fluorescent probe for highly selective detection of H 2 S in plant stress responses.

Authors :
Yang X
Zhang S
Luo F
Zhang Y
Yan D
Lai M
Ye Y
Sun K
Ji X
Source :
Biosensors & bioelectronics [Biosens Bioelectron] 2025 Jan 01; Vol. 267, pp. 116798. Date of Electronic Publication: 2024 Sep 19.
Publication Year :
2025

Abstract

Hydrogen sulfide (H <subscript>2</subscript> S) has emerged as a crucial signaling molecule in plant stress responses, playing a significant role in regulating various physiological and biochemical processes. In this study, we report an aggregation-induced emission (AIE)-based ratiometric fluorescent probe TPN-H <subscript>2</subscript> S for the highly selective detection of H <subscript>2</subscript> S in plant tissues. The probe exhibited excellent sensitivity and selectivity towards H <subscript>2</subscript> S over other analytes, enabling real-time monitoring of H <subscript>2</subscript> S dynamics in living cell. Furthermore, the AIE-based ratiometric probe TPN-H <subscript>2</subscript> S allowed for accurate quantification of H <subscript>2</subscript> S levels, providing valuable insights into the spatiotemporal distribution of Cys metabolism produces H <subscript>2</subscript> S. Importantly, the physiological pathways and signaling mechanisms of H <subscript>2</subscript> S production of was investigated in plant tissues under Cr and nano-plastics stress. Utilizing a high-throughput screening approach, we identified exogenous substances such as calcium chloride (CaCl <subscript>2</subscript> ) and abscisic acid (ABA) that could induce higher level of H <subscript>2</subscript> S production during the stress response in plants. Overall, those findings demonstrate the potential of the AIE-based ratiometric fluorescent probe TPN-H <subscript>2</subscript> S as a powerful tool for unraveling the role of H <subscript>2</subscript> S in plant stress responses and pave the way for further exploration of H <subscript>2</subscript> S-mediated signaling pathways in plants.<br />Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br /> (Copyright © 2024 Elsevier B.V. All rights reserved.)

Details

Language :
English
ISSN :
1873-4235
Volume :
267
Database :
MEDLINE
Journal :
Biosensors & bioelectronics
Publication Type :
Academic Journal
Accession number :
39312836
Full Text :
https://doi.org/10.1016/j.bios.2024.116798