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Soluble trehalase responds to heavy metal stimulation by regulating apoptosis in Neocaridina denticulata sinensis.
- Source :
-
Ecotoxicology and environmental safety [Ecotoxicol Environ Saf] 2024 Oct 15; Vol. 285, pp. 117072. Date of Electronic Publication: 2024 Sep 19. - Publication Year :
- 2024
-
Abstract
- Trehalase plays an important role in insect metabolism and development by hydrolyzing blood sugar trehalose, but it seems to perform primarily an immunomodulatory function in crustaceans whose blood sugar is glucose. Metal ions as pollutants seriously affecting crustacean health, but studies on trehalase in metal immunity are still limited. In this study, a soluble trehalase (NdTre1) that could bind to multiple metals was identified from Neocaridina denticulata sinensis for investigating metal resistance. Expression profiling revealed that NdTre1 was mainly expressed in the gill and was significantly decreased following stimulation with copper (Cu²⁺) and cadmium (Cd²⁺). Transcriptomic analysis of gills revealed an increase in ecdysone synthesis after interference with NdTre1. Increased ecdysone activated the endogenous mitochondrial pathway and the mitogen activated protein kinase (MAPK) pathway to further induced apoptosis. In vitro, Escherichia coli overexpressing recombinant NdTre1 had higher survival and faster growth rates to better adapted the metal-containing medium. Overall, NdTre1 exercises an important immune function in shrimp resistance to metal stimulation by regulating apoptosis and molting. Further investigation can further explore specific response mechanisms of NdTre1 to multiple metals.<br />Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br /> (Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Water Pollutants, Chemical toxicity
Gills drug effects
Ecdysone
Copper toxicity
Decapoda drug effects
Decapoda physiology
Decapoda genetics
Arthropod Proteins genetics
Arthropod Proteins metabolism
Molting drug effects
Gene Expression Profiling
Trehalase genetics
Trehalase metabolism
Apoptosis drug effects
Metals, Heavy toxicity
Cadmium toxicity
Subjects
Details
- Language :
- English
- ISSN :
- 1090-2414
- Volume :
- 285
- Database :
- MEDLINE
- Journal :
- Ecotoxicology and environmental safety
- Publication Type :
- Academic Journal
- Accession number :
- 39303639
- Full Text :
- https://doi.org/10.1016/j.ecoenv.2024.117072