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A Surface-Mediated Biomimetic Porous Polyether-Ether-Ketone Scaffold for Regulating Immunity and Promoting Osteogenesis.
- Source :
-
ACS biomaterials science & engineering [ACS Biomater Sci Eng] 2024 Oct 14; Vol. 10 (10), pp. 6120-6134. Date of Electronic Publication: 2024 Sep 18. - Publication Year :
- 2024
-
Abstract
- The repair of critical-sized bone defects remains a major challenge for clinical orthopedic surgery. Here, we develop a surface biofunctionalized three-dimensional (3D) porous polyether-ether-ketone (PEEK) scaffold that can simultaneously promote osteogenesis and regulate macrophage polarization. The scaffold is created using polydopamine (PDA)-assisted immobilization of silk fibroin (SF) and the electrostatic self-assembly of nanocrystalline hydroxyapatite (nano-HA) on a 3D-printed porous PEEK scaffold. The SF/nano-HA functionalized surface provides a bone-like microenvironment for osteoblastic cells' adhesion, proliferation, mineralization and osteogenic differentiation. Moreover, the biofunctionalized surface can effectively drive macrophages polarization from the pro-inflammatory M1 phenotype to the anti-inflammatory M2 phenotype. Integrin β1-specific cell-matrix binding and the activation of Ca <superscript>2+</superscript> receptor-mediated signaling pathway play critical roles in the regulation of macrophage polarization. Compared with the as-printed scaffold, the SF/nano-HA functionalized porous PEEK scaffold induces minimal inflammatory response, enhanced angiogenesis, and substantial new bone formation, resulting in improved osseointegration in vivo . This study not only develops a promising candidate for bone repair but also demonstrates a facile surface biofunctionalization strategy for orthopedic implants to improve osseointegration by stimulating osteogenesis and regulating immunity.
- Subjects :
- Animals
Porosity
Mice
Fibroins chemistry
Macrophages drug effects
Macrophages immunology
Durapatite chemistry
Biomimetic Materials chemistry
Biomimetic Materials pharmacology
RAW 264.7 Cells
Printing, Three-Dimensional
Surface Properties
Osteoblasts drug effects
Cell Differentiation drug effects
Indoles chemistry
Cell Adhesion drug effects
Osseointegration drug effects
Osteogenesis drug effects
Benzophenones
Polymers chemistry
Tissue Scaffolds chemistry
Polyethylene Glycols chemistry
Ketones chemistry
Ketones pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 2373-9878
- Volume :
- 10
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- ACS biomaterials science & engineering
- Publication Type :
- Academic Journal
- Accession number :
- 39295122
- Full Text :
- https://doi.org/10.1021/acsbiomaterials.4c00725