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Potential of Coffee Cherry Pulp Extract against Polycyclic Aromatic Hydrocarbons in Air Pollution Induced Inflammation and Oxidative Stress for Topical Applications.
- Source :
-
International journal of molecular sciences [Int J Mol Sci] 2024 Aug 30; Vol. 25 (17). Date of Electronic Publication: 2024 Aug 30. - Publication Year :
- 2024
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Abstract
- Airborne particulate matter (PM) contains polycyclic aromatic hydrocarbons (PAHs) as primary toxic components, causing oxidative damage and being associated with various inflammatory skin pathologies such as premature aging, atopic dermatitis, and psoriasis. Coffee cherry pulp (CCS) extract, rich in chlorogenic acid, caffeine, and theophylline, has demonstrated strong antioxidant properties. However, its specific anti-inflammatory effects and ability to protect macrophages against PAH-induced inflammation remain unexplored. Thus, this study aimed to evaluate the anti-inflammatory properties of CCS extract on RAW 264.7 macrophage cells exposed to atmospheric PAHs, compared to chlorogenic acid (CGA), caffeine (CAF), and theophylline (THP) standards. The CCS extract was assessed for its impact on the production of nitric oxide (NO) and expression of tumor necrosis factor-alpha (TNF- α ), interleukin-6 (IL-6), inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2). Results showed that CCS extract exhibited significant antioxidant activities and effectively inhibited protease and lipoxygenase (LOX) activities. The PAH induced the increase in intracellular reactive oxygen species, NO, TNF- α , IL-6, iNOS, and COX-2, which were markedly suppressed by CCS extract in a dose-dependent manner, comparable to the effects of chlorogenic acid, caffeine, and theophylline. In conclusion, CCS extract inhibits PAH-induced inflammation by reducing pro-inflammatory cytokines and reactive oxygen species (ROS) production in RAW 264.7 cells. This effect is likely due to the synergistic effects of its bioactive compounds. Chlorogenic acid showed strong antioxidant and anti-inflammatory activities, while caffeine and theophylline enhanced anti-inflammatory activity. CCS extract did not irritate the hen's egg chorioallantoic membrane. Therefore, CCS extract shows its potential as a promising cosmeceutical ingredient for safely alleviating inflammatory skin diseases caused by air pollution.
- Subjects :
- Animals
Mice
RAW 264.7 Cells
Inflammation drug therapy
Inflammation chemically induced
Inflammation metabolism
Antioxidants pharmacology
Reactive Oxygen Species metabolism
Nitric Oxide metabolism
Air Pollution adverse effects
Nitric Oxide Synthase Type II metabolism
Macrophages drug effects
Macrophages metabolism
Chlorogenic Acid pharmacology
Administration, Topical
Tumor Necrosis Factor-alpha metabolism
Coffea chemistry
Caffeine pharmacology
Particulate Matter toxicity
Oxidative Stress drug effects
Plant Extracts pharmacology
Plant Extracts chemistry
Polycyclic Aromatic Hydrocarbons toxicity
Anti-Inflammatory Agents pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1422-0067
- Volume :
- 25
- Issue :
- 17
- Database :
- MEDLINE
- Journal :
- International journal of molecular sciences
- Publication Type :
- Academic Journal
- Accession number :
- 39273362
- Full Text :
- https://doi.org/10.3390/ijms25179416