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Sodium butyrate alleviates lipopolysaccharide-induced inflammation through JAK/STAT signalling in primary human corneal fibroblasts.
- Source :
-
European journal of pharmacology [Eur J Pharmacol] 2024 Nov 15; Vol. 983, pp. 176998. Date of Electronic Publication: 2024 Sep 11. - Publication Year :
- 2024
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Abstract
- Background: Bacterial keratitis is a common cause of blindness. Antibiotic treatment leads to the rapid release of lipopolysaccharide (LPS), which can activate corneal fibroblasts and cause persistent and excessive inflammatory responses. The anti-inflammatory drugs currently used to treat keratitis have serious side effects. Therefore, the ability of sodium butyrate (NaB), which can suppress the production of proinflammatory cytokines and promote the production of anti-inflammatory cytokines, to ameliorate keratitis was assessed in the present study.<br />Methods: The effect of NaB on the viability of primary human corneal fibroblasts was assayed with a CCK-8 kit. Cell migration was assessed by an in vitro scratch assay. Cell phenotypes were assessed by Western blotting and immunofluorescence staining. An antibody array was used to measure the production of proinflammatory cytokines and chemokines.<br />Results: At 0-1 mM, NaB had no significant effect on cell viability, promoted the expression of the keratocyte marker keratocan and inhibited the fibroblast marker vimentin. Inhibition of cell migration was observed in the wound healing assay. By targeting the Janus kinase/signal transducer and activator of transcription (JAK/STAT) signalling pathway, NaB decreased the levels of inflammation-related cytokines and chemokines whose expression was induced by LPS.<br />Conclusions: NaB maintained the keratocyte phenotype, inhibited cell migration, and relieved LPS-induced inflammatory responses through the JAK/STAT signalling pathway.<br />Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br /> (Copyright © 2024 Elsevier B.V. All rights reserved.)
- Subjects :
- Humans
Cytokines metabolism
Inflammation drug therapy
Inflammation pathology
Inflammation metabolism
Cells, Cultured
Cell Survival drug effects
Anti-Inflammatory Agents pharmacology
Anti-Inflammatory Agents therapeutic use
Lipopolysaccharides pharmacology
Butyric Acid pharmacology
Fibroblasts drug effects
Fibroblasts metabolism
Fibroblasts pathology
Signal Transduction drug effects
Janus Kinases metabolism
Cell Movement drug effects
Cornea drug effects
Cornea pathology
Cornea metabolism
STAT Transcription Factors metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0712
- Volume :
- 983
- Database :
- MEDLINE
- Journal :
- European journal of pharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 39271038
- Full Text :
- https://doi.org/10.1016/j.ejphar.2024.176998