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Functional impacts of PtrMYB203 on phenylpropanoid pathway regulation and wood properties in hybrid poplar.

Authors :
Cho JS
Kim MH
Jang HA
Choi H
Jeon HW
Lee H
Ko JH
Source :
Plant physiology and biochemistry : PPB [Plant Physiol Biochem] 2024 Sep 12; Vol. 216, pp. 109118. Date of Electronic Publication: 2024 Sep 12.
Publication Year :
2024
Publisher :
Ahead of Print

Abstract

The phenylpropanoid pathway is vital for plant growth and development, producing lignin and flavonoids. This study investigates PtrMYB203, a homolog of MYB repressors of proanthocyanidin (PA) biosynthesis in Populus trichocarpa, as a transcriptional repressor in the phenylpropanoid pathway of hybrid poplar (Populus alba x P. glandulosa). Overexpression of PtrMYB203 (35S::PtrMYB203) in hybrid poplar detrimentally impacted plant growth and development. Histological analysis revealed irregular xylem vessel formation and decreased lignin content, corroborated by Klason lignin assays. Moreover, 35S::PtrMYB203 transgenic poplars exhibited significant decreases in anthocyanin and PA accumulations in callus tissues, even under high light conditions. Quantitative RT-PCR analysis and protoplast-based transcriptional activation assay confirmed the downregulation of lignin and flavonoid biosynthesis genes. This genetic modification also alters the expression of several MYB transcription factors, essential for phenylpropanoid pathway regulation. Remarkably, saccharification efficiency in the 35S::PtrMYB203 poplar was improved by over 34% following hot water treatment alone. These findings suggest PtrMYB203 as a potential genetic target for enhancing wood properties for bioenergy production, providing valuable insights into the manipulation of metabolite pathways in woody perennials to advance wood biotechnology.<br />Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br /> (Copyright © 2024 Elsevier Masson SAS. All rights reserved.)

Details

Language :
English
ISSN :
1873-2690
Volume :
216
Database :
MEDLINE
Journal :
Plant physiology and biochemistry : PPB
Publication Type :
Academic Journal
Accession number :
39270565
Full Text :
https://doi.org/10.1016/j.plaphy.2024.109118