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Segmental patterning of microbiota and immune cells in the murine intestinal tract.
- Source :
-
Gut microbes [Gut Microbes] 2024 Jan-Dec; Vol. 16 (1), pp. 2398126. Date of Electronic Publication: 2024 Sep 10. - Publication Year :
- 2024
-
Abstract
- The intestine exhibits distinct characteristics along its length, with a substantial immune cell reservoir and diverse microbiota crucial for maintaining health. This study investigates how anatomical location and regional microbiota influence intestinal immune cell abundance. Using conventionally colonized and germ-free mice, segment-specific immune cell composition and microbial communities were assessed. Metagenomic sequencing analyzed microbiome variations, while flow cytometry and immunofluorescence examined immune cell composition. Microbiome composition varied significantly along the intestine, with diversity and abundance increasing from upper to lower segments. Immune cells showed distinct segment-specific patterning influenced by microbial colonization and localization. T cell subsets displayed varied dependence on microbiome presence and anatomical location. This study highlights locoregional differences in intestinal immune cell and microbiome composition, identifying immune subsets susceptible to microbiota presence. The findings provide context for understanding immune cell alterations in disease models.
- Subjects :
- Animals
Mice
Intestines microbiology
Intestines immunology
Intestines cytology
Metagenomics
Germ-Free Life
Female
T-Lymphocyte Subsets immunology
Male
Intestinal Mucosa microbiology
Intestinal Mucosa immunology
Intestinal Mucosa cytology
Gastrointestinal Microbiome
Mice, Inbred C57BL
Bacteria classification
Bacteria genetics
Bacteria isolation & purification
Bacteria immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1949-0984
- Volume :
- 16
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Gut microbes
- Publication Type :
- Academic Journal
- Accession number :
- 39254265
- Full Text :
- https://doi.org/10.1080/19490976.2024.2398126