Target-induced enzymatic cleavage cycle amplification reaction-gated organic photoelectrochemical transistor biosensor for rapid detection of okadaic acid.

Okadaic acid (OA), a predominant toxic entity in Diarrhetic Shellfish Poisoning (DSP), carries substantial significance for both marine ecosystems and human well-being. The nascent organic photoelectrochemical transistor (OPECT) biosensor has emerged as a promising biometric methodology, poised to offer a fresh realm for the detection of marine biotoxins. In this work, a biosensor utilizing signal amplification based on Cd _0.5 Zn _0.5 S/ZnIn ₂ S ₄ quantum dots (CZS/ZIS QDs) in OPECT was proposed for OA detection, where ZIS QDs were labeled on aptamer and a substantial quantity of QDs were generated via cyclic shearing facilitated through target-induced Exo I enzyme. Owing to the sensitizing influence of ZIS QDs on CZS, the photoelectric conversion efficiency was augmented, culminating in a notable anodic photocurrent upon exposure to light, thereby inducing a transformation in the channel state of the polymer poly(3,4-ethylenedioxythiophene): poly(styrene sulfonate) and consequently producing a remarkable modification in the channel current. The detection limit of the biosensor as low as 12.5 pM and a superior stability and specificity was confirmed, which also showed commendable outcomes in actual samples testing. Consequently, this study not only introduces a novel pathway for swift OA detection, but unveils a novel perspective for future expedited and convenient on-site detection of marine biotoxins.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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