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Synthesis of Pyrazolo[3,4-b]pyridine Derivatives and Their In-Vitro and In-Silico Antidiabetic Activities.
- Source :
-
Journal of cellular biochemistry [J Cell Biochem] 2024 Sep 06, pp. e30646. Date of Electronic Publication: 2024 Sep 06. - Publication Year :
- 2024
- Publisher :
- Ahead of Print
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Abstract
- In the current study, new pyrazolo[3,4-b]pyridine esters, hydrazides, and Schiff bases have been synthesized starting from 3-methyl-1-phenyl-1H-pyrazol-5-amine. The first step involved solvent-free synthesis of pyrazolo[3,4-b]pyridine-6-carboxylate derivatives (2a-d) with 55%-70% yield in the minimum time frame compared with the conventional refluxing method, which was followed by the synthesis of corresponding hydrazides (3a-d) and hydrazones (4a-e). The structures of the synthesized derivatives were confirmed using element analysis, FT-IR, <superscript>1</superscript> H NMR, <superscript>13</superscript> C NMR, and LC-MS techniques. Synthesized hydrazides (3a-d) and hydrazones (4a-e) were also tested for their in-vitro antidiabetic activity and found that all the compounds exhibited significant antidiabetic activity, while 3c (IC <subscript>50</subscript> = 9.6 ± 0.5 μM) among the hydrazides and 4c (IC <subscript>50</subscript> = 13.9 ± 0.7 μM) among the hydrazones were found to be more active in comparison to other synthesized derivatives. These in-vitro results were further validated via docking studies against the α-amylase enzyme using the reference drug acarbose (200.1 ± 10.0 μM). The results were greatly in agreement with their in-vitro studies and these derivatives can be encouraging candidates for further in-vivo studies in mice models.<br /> (© 2024 Wiley Periodicals LLC.)
Details
- Language :
- English
- ISSN :
- 1097-4644
- Database :
- MEDLINE
- Journal :
- Journal of cellular biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 39239805
- Full Text :
- https://doi.org/10.1002/jcb.30646