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lncRNA H19 facilitates vascular neointima formation by targeting miR-125a-3p/FLT1 axis.
- Source :
-
Acta biochimica et biophysica Sinica [Acta Biochim Biophys Sin (Shanghai)] 2024 Sep 02; Vol. 56 (10), pp. 1437-1445. - Publication Year :
- 2024
-
Abstract
- The aberrant proliferation and migration of vascular smooth muscle cells (VSMCs) contribute to the development of neointima formation in vascular restenosis. This study aims to explore the function of the long noncoding RNA H19 in neointima formation. A mouse carotid ligation model was established, and human vascular smooth muscle cells (VSMCs) were used as a cell model. lncRNA H19 overexpression promoted VSMC proliferation and migration. Moreover, miR-125a-3p potentially bound to lncRNA H19, and Fms-like tyrosine kinase-1 (FLT1) might be a direct target of miR-125a-3p in VSMCs. Upregulation of miR-125a-3p alleviated lncRNA H19-enhanced VSMC proliferation and migration. Furthermore, rescue experiments showed that enhanced expression of miR-125a-3p attenuated lncRNA H19-induced FLT1 expression in VSMCs. In addition, the overexpression of lncRNA H19 significantly exacerbated neointima formation in a mouse carotid ligation model. In summary, lncRNA H19 stimulates VSMC proliferation and migration by acting as a competing endogenous RNA (ceRNA) of miR-125a-3p. lncRNA H19 may be a therapeutic target for restenosis.
- Subjects :
- Animals
Humans
Mice
Vascular Endothelial Growth Factor Receptor-1 genetics
Vascular Endothelial Growth Factor Receptor-1 metabolism
Myocytes, Smooth Muscle metabolism
Myocytes, Smooth Muscle pathology
Mice, Inbred C57BL
Male
Cells, Cultured
RNA, Long Noncoding genetics
RNA, Long Noncoding metabolism
MicroRNAs genetics
MicroRNAs metabolism
Neointima pathology
Neointima metabolism
Neointima genetics
Cell Proliferation genetics
Cell Movement genetics
Muscle, Smooth, Vascular metabolism
Muscle, Smooth, Vascular pathology
Muscle, Smooth, Vascular cytology
Subjects
Details
- Language :
- English
- ISSN :
- 1745-7270
- Volume :
- 56
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Acta biochimica et biophysica Sinica
- Publication Type :
- Academic Journal
- Accession number :
- 39238439
- Full Text :
- https://doi.org/10.3724/abbs.2024087