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Preparation of viromimetic rod-like nanoparticle vaccines (RLNVax) and study of their humoral immune activation efficacy.
- Source :
-
Biomaterials science [Biomater Sci] 2024 Sep 25; Vol. 12 (19), pp. 5115-5122. Date of Electronic Publication: 2024 Sep 25. - Publication Year :
- 2024
-
Abstract
- Virus-like nanoparticle vaccines can efficiently activate the humoral immune response by cross-linking B cell receptors with their surface multivalent antigen arrays. This structurally dependent mechanism makes it crucial to regulate and optimize structural parameters to enhance the efficacy of nanoparticle vaccines. In this study, we prepared nanoparticle vaccines with different aspect ratios by chemically modifying antigen proteins onto the surfaces of poly(amino acid) nanoparticles of various shapes (spherical, ellipsoidal, and rod-like). This allowed us to investigate the impact of structural anisotropy on the humoral immune activation efficacy of nanoparticle vaccines. Furthermore, the end-group molecules of poly(amino acid) materials possess aggregation-induced emission (AIE) properties, which facilitate monitoring the dynamics of nano-assemblies within the body. Results showed that rod-like nanoparticle vaccines (RLNVax) with a higher aspect ratio (AR = 5) exhibited greater lymph node draining efficiency and could elicit more effective B cell activation compared to conventional isotropic spherical nanoparticle vaccines. In a murine subcutaneous immunization model using ovalbumin (OVA) as a model antigen, RLNVax elicited antigen-specific antibody titers that were about 64 times and 4.6 times higher than those induced by free antigen proteins and spherical nanoparticle vaccines, respectively. Additionally, when combined with an aluminum adjuvant, antibody titers elicited by RLNVax were further enhanced by 4-fold. These findings indicate that the anisotropic rod-like structure is advantageous for improving the humoral immune activation efficacy of nanoparticle vaccines, providing significant insights for the design and optimization of next-generation nanoparticle vaccines.
- Subjects :
- Animals
Mice
Female
Ovalbumin immunology
Ovalbumin chemistry
Ovalbumin administration & dosage
Mice, Inbred BALB C
B-Lymphocytes immunology
Vaccines, Virus-Like Particle immunology
Vaccines, Virus-Like Particle chemistry
Vaccines, Virus-Like Particle administration & dosage
Nanovaccines
Immunity, Humoral drug effects
Nanoparticles chemistry
Nanoparticles administration & dosage
Subjects
Details
- Language :
- English
- ISSN :
- 2047-4849
- Volume :
- 12
- Issue :
- 19
- Database :
- MEDLINE
- Journal :
- Biomaterials science
- Publication Type :
- Academic Journal
- Accession number :
- 39225616
- Full Text :
- https://doi.org/10.1039/d4bm00827h