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GhJUB1_3-At positively regulate drought and salt stress tolerance under control of GhHB7, GhRAP2-3 and GhRAV1 in Cotton.
- Source :
-
Physiologia plantarum [Physiol Plant] 2024 Sep-Oct; Vol. 176 (5), pp. e14497. - Publication Year :
- 2024
-
Abstract
- Climate change severely affects crop production. Cotton is one of the primary fiber crops in the world and its production is susceptible to various environmental stresses, especially drought and salinity. Development of stress tolerant genotypes is the only way to escape from these environmental constraints. We identified sixteen homologs of the Arabidopsis JUB1 gene in cotton. Expression of GhJUB1&#95;3-At was significantly induced in the temporal expression analysis of GhJUB1 genes in the roots of drought tolerant (H177) and susceptible (S9612) cotton genotypes under drought. The silencing of the GhJUB1&#95;3-At gene alone and together with its paralogue GhJUB1&#95;3-Dt reduced the drought tolerance in cotton plants. The transgenic lines exhibited tolerance to the drought and salt stress as compared to the wildtype (WT). The chlorophyll and relative water contents of wildtype decreased under drought as compared to the transgenic lines. The transgenic lines showed decreased H <subscript>2</subscript> O <subscript>2</subscript> and increased proline levels under drought and salt stress, as compared to the WT, indicating that the transgenic lines have drought and salt stress tolerance. The expression analysis of the transgenic lines and WT revealed that GAI was upregulated in the transgenic lines in normal conditions as compared to the WT. Under drought and salt treatment, RAB18 and RD29A were strongly upregulated in the transgenic lines as compared to the WT. Conclusively, GhJUB1&#95;3-At is not an auto activator and it is regulated by the crosstalk of GhHB7, GhRAP2-3 and GhRAV1. GhRAV1, a negative regulator of abiotic stress tolerance and positive regulator of leaf senescence, suppresses the expression of GhJUB1&#95;3-At under severe circumstances leading to plant death.<br /> (© 2024 Scandinavian Plant Physiology Society.)
- Subjects :
- Stress, Physiological genetics
Salt Stress genetics
Salt Stress physiology
Arabidopsis genetics
Arabidopsis physiology
Gossypium genetics
Gossypium physiology
Gossypium metabolism
Droughts
Gene Expression Regulation, Plant
Plant Proteins genetics
Plant Proteins metabolism
Plants, Genetically Modified
Salt Tolerance genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1399-3054
- Volume :
- 176
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Physiologia plantarum
- Publication Type :
- Academic Journal
- Accession number :
- 39223909
- Full Text :
- https://doi.org/10.1111/ppl.14497