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Metabolomics-based profiling of anti-inflammatory compounds from Mentha spicata in shanghe, China.

Metabolomics-based profiling of anti-inflammatory compounds from Mentha spicata in shanghe, China.

Authors :
Li W
Li P
Li X
Hou H
Lin H
Jin M
Liu K
Zhang X
Sheng W
Source :
Heliyon [Heliyon] 2024 Aug 08; Vol. 10 (16), pp. e35974. Date of Electronic Publication: 2024 Aug 08 (Print Publication: 2024).
Publication Year :
2024

Abstract

Mentha spicata is a popular herb used in foods, cosmetics, and medicines. In the present study, liquid chromatography-mass spectrometry-based metabolomics analysis and the zebrafish model were used to investigate the potential biomarkers of M. spicata growing in Shanghe County (Shandong Province, China) and their anti-inflammatory properties. Network pharmacology and molecular docking were performed to screen the main targets of the characteristic compounds to understand their mechanisms of action. Nine potential markers including sugars ( 1 , 2 ), polyphenolic acids ( 3 - 5 ), and flavonoids ( 6 - 9 ) were identified from the species. The inhibitory effects on leukocyte migration confirmed that compounds 1 and 3 - 9 played a positive role in the protective effect of Shanghe M. spicata (SM) extract against inflammation. Akt (protein kinase B), EGFR (epidermal growth factor receptor), and MMP9 (matrix metalloproteinase 9) were the core target proteins of the identified compounds in the anti-inflammatory process. The most significant Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment terms were response to abiotic stimulus (Biological Process), carbohydrate derivative binding (Molecular Function), and pathways in cancer. In docking simulations, 3-p-coumaroylquinic acid (3-PC, 4 ) and cirsimaritin (CN, 7 ) exhibited the highest potential affinity to the active sites of Akt and EGFR proteins, respectively; additionally, 5-demethylsinensetin (5-DS, 9 ) and luteolin (LN, 6 ) were considered the most suitable ligands for the MMP9 protein. The present study highlighted the use of SM resources as functional products with health benefits.<br />Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br /> (© 2024 The Authors. Published by Elsevier Ltd.)

Details

Language :
English
ISSN :
2405-8440
Volume :
10
Issue :
16
Database :
MEDLINE
Journal :
Heliyon
Publication Type :
Academic Journal
Accession number :
39220934
Full Text :
https://doi.org/10.1016/j.heliyon.2024.e35974