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ZmGDIα-hel counters the RBSDV-induced reduction of active gibberellins to alleviate maize rough dwarf virus disease.
- Source :
-
Nature communications [Nat Commun] 2024 Aug 31; Vol. 15 (1), pp. 7576. Date of Electronic Publication: 2024 Aug 31. - Publication Year :
- 2024
-
Abstract
- Maize rough dwarf disease (MRDD) threatens maize production globally. The P7-1 effector of the rice black-streaked dwarf virus (RBSDV) targets maize Rab GDP dissociation inhibitor alpha (ZmGDIα) to cause MRDD. However, P7-1 has difficulty recruiting a ZmGDIα variant with an alternative helitron-derived exon 10 (ZmGDIα-hel), resulting in recessive resistance. Here, we demonstrate that P7-1 can recruit another maize protein, gibberellin 2-oxidase 13 (ZmGA2ox7.3), which also exhibits tighter binding affinity for ZmGDIα than ZmGDIα-hel. The oligomerization of ZmGA2ox7.3 is vital for its function in converting bioactive gibberellins into inactive forms. Moreover, the enzymatic activity of ZmGA2ox7.3 oligomers increases when forming hetero-oligomers with P7-1/ZmGDIα, but decreases when ZmGDIα-hel replaces ZmGDIα. Viral infection significantly promotes ZmGA2ox7.3 expression and oligomerization in ZmGDIα-containing susceptible maize, resulting in reduced bioactive GA <subscript>1</subscript> /GA <subscript>4</subscript> levels. This causes an auxin/cytokinin imbalance and ultimately manifests as MRDD syndrome. Conversely, in resistant maize, ZmGDIα-hel counters these virus-induced changes, thereby mitigating MRDD severity.<br /> (© 2024. The Author(s).)
- Subjects :
- Plant Viruses physiology
Disease Resistance
Gene Expression Regulation, Plant
Plants, Genetically Modified
Viral Proteins metabolism
Viral Proteins genetics
Zea mays virology
Zea mays metabolism
Plant Diseases virology
Gibberellins metabolism
Plant Proteins metabolism
Plant Proteins genetics
Subjects
Details
- Language :
- English
- ISSN :
- 2041-1723
- Volume :
- 15
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Nature communications
- Publication Type :
- Academic Journal
- Accession number :
- 39217146
- Full Text :
- https://doi.org/10.1038/s41467-024-51726-7