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Polypeptide N-acetylgalactosaminyltransferase-15 regulates adipogenesis in human SGBS cells.
- Source :
-
Scientific reports [Sci Rep] 2024 Aug 29; Vol. 14 (1), pp. 20049. Date of Electronic Publication: 2024 Aug 29. - Publication Year :
- 2024
-
Abstract
- Adipogenesis involves intricate molecular mechanisms regulated by various transcription factors and signaling pathways. In this study, we aimed to identify factors specifically induced during adipogenesis in the human preadipocyte cell line, SGBS, but not in the mouse preadipocyte cell line, 3T3-L1. Microarray analysis revealed distinct gene expression profiles, with 1460 genes induced in SGBS cells and 1297 genes induced in 3T3-L1 cells during adipogenesis, with only 297 genes commonly induced. Among the genes uniquely induced in SGBS cells, we focused on GALNT15, which encodes polypeptide N-acetylgalactosaminyltransferase-15. Its expression increased transiently during adipogenesis in SGBS cells but remained low in 3T3-L1 cells. Overexpression of GALNT15 increased mRNA levels of CCAAT-enhancer binding protein (C/EBPα) and leptin but had no significant impact on adipogenesis in SGBS cells. Conversely, knockdown of GALNT15 suppressed mRNA expression of adipocyte marker genes, reduced lipid accumulation, and decreased the percentage of cells with oil droplets. The induction of C/EBPα and peroxisome proliferator-activated receptor γ during adipogenesis was promoted or suppressed in SGBS cells subjected to overexpression or knockdown of GALNT15, respectively. These data suggest that polypeptide N-acetylgalactosaminyltransferase-15 is a novel regulatory molecule that enhances adipogenesis in SGBS cells.<br /> (© 2024. The Author(s).)
- Subjects :
- Humans
Mice
Animals
Adipocytes metabolism
Adipocytes cytology
PPAR gamma metabolism
PPAR gamma genetics
Cell Line
CCAAT-Enhancer-Binding Proteins metabolism
CCAAT-Enhancer-Binding Proteins genetics
Leptin metabolism
Leptin genetics
Adipogenesis genetics
N-Acetylgalactosaminyltransferases metabolism
N-Acetylgalactosaminyltransferases genetics
Polypeptide N-acetylgalactosaminyltransferase
3T3-L1 Cells
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 14
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 39209927
- Full Text :
- https://doi.org/10.1038/s41598-024-70930-5