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Internal structure of Mycoplasma mobile gliding machinery analyzed by negative staining electron tomography.
- Source :
-
Biophysics and physicobiology [Biophys Physicobiol] 2024 May 28; Vol. 21 (2), pp. e210015. Date of Electronic Publication: 2024 May 28 (Print Publication: 2024). - Publication Year :
- 2024
-
Abstract
- Mycoplasma mobile is a parasitic bacterium that forms gliding machinery on the cell pole and glides on a solid surface in the direction of the cell pole. The gliding machinery consists of both internal and surface structures. The internal structure is divided into a bell at the front and chain structure extending from the bell. In this study, the internal structures prepared under several conditions were analyzed using negative-staining electron microscopy and electron tomography. The chains were constructed by linked motors containing two complexes similar to ATP synthase. A cylindrical spacer with a maximum diameter of 6 nm and a height of 13 nm, and anonymous linkers with a diameter of 0.9-8.3 nm and length of 14.7±6.9 nm were found between motors. The bell is bowl-shaped and features a honeycomb surface with a periodicity of 8.4 nm. The chains of the motor are connected to the rim of the bell through a wedge-shaped structure. These structures may play roles in the assembly and cooperation of gliding machinery units.<br />Competing Interests: The authors declare that they have no conflict of interest.<br /> (2024 THE BIOPHYSICAL SOCIETY OF JAPAN.)
Details
- Language :
- English
- ISSN :
- 2189-4779
- Volume :
- 21
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Biophysics and physicobiology
- Publication Type :
- Academic Journal
- Accession number :
- 39206130
- Full Text :
- https://doi.org/10.2142/biophysico.bppb-v21.0015