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Puerarin Alleviates Blood Pressure via Inhibition of ROS/TLR4/NLRP3 Inflammasome Signaling Pathway in the Hypothalamic Paraventricular Nucleus of Salt-Induced Prehypertensive Rats.
- Source :
-
Nutrients [Nutrients] 2024 Aug 06; Vol. 16 (16). Date of Electronic Publication: 2024 Aug 06. - Publication Year :
- 2024
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Abstract
- Background: Puerarin is an isoflavone compound isolated from the roots of a leguminous plant, the wild kudzu. Various functional activities of this compound in multiple diseases have been reported. However, the effect and mechanism of puerarin in improving blood pressure remain non-elucidated.<br />Purpose: The current study was designed to assess the preventive effects of puerarin on the onset and progression of hypertension and to verify the hypothesis that puerarin alleviates blood pressure by inhibiting the ROS/TLR4/NLRP3 inflammasome signaling pathway in the hypothalamic paraventricular nucleus (PVN) of salt-induced prehypertensive rats.<br />Methods: Male Dahl salt-sensitive rats were fed low NaCl salt (3% in drinking water) for the control (NS) group or 8% (HS) to induce prehypertension. Each batch was divided into two group and treated by bilateral PVN microinjection with either artificial cerebrospinal fluid or puerarin through a micro-osmotic pump for 6 weeks. The mean arterial pressure (MAP) was recorded, and samples were collected and analyzed.<br />Results: We concluded that puerarin significantly prevented the elevation of blood pressure and effectively alleviated the increase in heart rate caused by high salt. Norepinephrine (NE) in the plasma of salt-induced prehypertensive rats also decreased upon puerarin chronic infusion. Additionally, analysis of the PVN sample revealed that puerarin pretreatment decreased the positive cells and gene level of TLR4 (Toll-like receptor 4), NLRP3, Caspase-1 p10, NOX2, MyD88, NOX4, and proinflammatory cytokines in the PVN. Puerarin pretreatment also decreased NF-κBp65 activity, inhibited oxidative stress, and alleviated inflammatory responses in the PVN.<br />Conclusion: We conclude that puerarin alleviated blood pressure via inhibition of the ROS/TLR4/NLRP3 inflammasome signaling pathway in the PVN, suggesting the therapeutic potential of puerarin in the prevention of hypertension.
- Subjects :
- Animals
Male
Rats
Disease Models, Animal
Hypertension chemically induced
Hypertension drug therapy
Prehypertension drug therapy
Rats, Inbred Dahl
Sodium Chloride, Dietary
Blood Pressure drug effects
Inflammasomes metabolism
Inflammasomes drug effects
Isoflavones pharmacology
NLR Family, Pyrin Domain-Containing 3 Protein metabolism
Paraventricular Hypothalamic Nucleus metabolism
Paraventricular Hypothalamic Nucleus drug effects
Reactive Oxygen Species metabolism
Signal Transduction drug effects
Toll-Like Receptor 4 metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2072-6643
- Volume :
- 16
- Issue :
- 16
- Database :
- MEDLINE
- Journal :
- Nutrients
- Publication Type :
- Academic Journal
- Accession number :
- 39203718
- Full Text :
- https://doi.org/10.3390/nu16162580