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The embryonic zebrafish brain is exclusively colonized by pu.1 -dependent and lymphatic-independent population of microglia.
- Source :
-
Science advances [Sci Adv] 2024 Aug 30; Vol. 10 (35), pp. eado0519. Date of Electronic Publication: 2024 Aug 28. - Publication Year :
- 2024
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Abstract
- Microglia, the crucial immune cells inhabiting the central nervous system (CNS), perform a range of vital functions, encompassing immune defense and neuronal regulation. Microglia subsets with diverse functions and distinct developmental regulations have been identified recently. It is generally accepted that all microglia originate from hematopoiesis and depend on the myeloid transcription factor PU.1. However, a recent study reported the existence of mrc1 <superscript>+</superscript> microglia in zebrafish embryos, which are seemingly independent of Pu.1 and reliant on lymphatic vessels, sparking great interest in the possibility of lymphatic-originated microglia. To address this, we took advantage of a pu.1 knock-in zebrafish allele for a detailed investigation. Our results conclusively showed that almost all zebrafish embryonic microglia (~95% on average) express pu.1 . Further, lineage tracing and mutant analysis revealed that these microglia neither emerged from nor depended on lymphatic vessels. In essence, our study refutes the presence of pu.1 -independent but lymphatic-dependent microglia.
- Subjects :
- Animals
Zebrafish Proteins genetics
Zebrafish Proteins metabolism
Embryo, Nonmammalian metabolism
Microglia metabolism
Zebrafish embryology
Trans-Activators metabolism
Trans-Activators genetics
Proto-Oncogene Proteins metabolism
Proto-Oncogene Proteins genetics
Brain metabolism
Brain embryology
Lymphatic Vessels metabolism
Lymphatic Vessels cytology
Lymphatic Vessels embryology
Subjects
Details
- Language :
- English
- ISSN :
- 2375-2548
- Volume :
- 10
- Issue :
- 35
- Database :
- MEDLINE
- Journal :
- Science advances
- Publication Type :
- Academic Journal
- Accession number :
- 39196933
- Full Text :
- https://doi.org/10.1126/sciadv.ado0519