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Infection by a multidrug-resistant Corynebacterium diphtheriae strain: prediction of virulence factors, CRISPR-Cas system analysis, and structural implications of mutations conferring rifampin resistance.
- Source :
-
Functional & integrative genomics [Funct Integr Genomics] 2024 Aug 28; Vol. 24 (5), pp. 145. Date of Electronic Publication: 2024 Aug 28. - Publication Year :
- 2024
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Abstract
- Cases of diphtheria, even in immunized individuals, are still reported in several parts of the world, including in Brazil. New outbreaks occur in Europe and other continents. In this context, studies on Corynebacterium diphtheriae infections are highly relevant, both for a better understanding of the pathogenesis of the disease and for controlling the circulation of clones and antimicrobial resistance genes. Here we present a case of cutaneous infection by multidrug-resistant Corynebacterium diphtheriae and provide its whole-genome sequencing. Genomic analysis revealed resistance genes, including tet(W), sul1, cmx, rpoB2, rbpA and mutation in rpoB. We performed phylogenetic analyzes and used the BRIG to compare the predicted resistance genes with those found in genomes from other significant isolates, including those associated with some outbreaks. Virulence factors such as spaD, srtBC, spaH, srtDE, surface-anchored pilus proteins (sapD), nonfimbrial adhesins (DIP0733, DIP1281, and DIP1621), embC and mptC (putatively involved in CdiLAM), sigA, dtxR and MdbA (putatively involved) in post-translational modification, were detected. We identified the CRISPR-Cas system in our isolate, which was classified as Type II-U based on the database and contains 15 spacers. This system functions as an adaptive immune mechanism. The strain was attributed to a new sequence type ST-928, and phylogenetic analysis confirmed that it was related to ST-634 of C. diphtheriae strains isolated in French Guiana and Brazil. In addition, since infections are not always reported, studies with the sequence data might be a way to complement and inform C. diphtheriae surveillance.<br /> (© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
- Subjects :
- Humans
Mutation
Phylogeny
Diphtheria microbiology
Genome, Bacterial
Bacterial Proteins genetics
Drug Resistance, Multiple, Bacterial genetics
Corynebacterium diphtheriae genetics
Corynebacterium diphtheriae pathogenicity
Corynebacterium diphtheriae drug effects
Virulence Factors genetics
CRISPR-Cas Systems
Rifampin pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1438-7948
- Volume :
- 24
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Functional & integrative genomics
- Publication Type :
- Academic Journal
- Accession number :
- 39196424
- Full Text :
- https://doi.org/10.1007/s10142-024-01434-8