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[Endothelial cells and fibroblasts mediate the microenvironmental regulatory network of carotid body paraganglioma].
- Source :
-
Lin chuang er bi yan hou tou jing wai ke za zhi = Journal of clinical otorhinolaryngology, head, and neck surgery [Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi] 2024 Sep; Vol. 38 (9), pp. 788-796. - Publication Year :
- 2024
-
Abstract
- Objective: To explore the gene expression characteristics of endothelial cells and fibroblasts in the microenvironment of SDHD -mutated carotid body tumors( SDHD -CBT), to fine the functional enrichment of each subcluster, and to further explore the network of cell-cell interactions in the microenvironment of SDHD -CBT. Methods: The bioinformatics analysis was used to download and reanalyze the single-nuclear RNA sequencing data of SDHD -CBT, SDHB mutated thoracic and abdominal paraganglioma( SDHB -ATPGL), SDHB -CBT, and normal adrenal medulla(NAM), to clarify the information of cell populations of the samples. We focused on exploring the gene expression profiles of endothelial cells and fibroblasts subclusters, and performed functional enrichment analysis based on Gene Ontology(GO) resources. CellChat was used to compare the cell-cell interactions networks of different clinical samples and predict significant signaling pathways in SDHD -CBT. Results: A total of 7 cell populations were profiled. The main subtypes of endothelial cells in SDHD -CBT are arterial and venous endothelial cells, and the main subtypes of fibroblasts are myofibroblasts and pericytes. Compared to NAM, SDHB -CBT and SDHB -ATPGL, cell communication involving endothelial cells and fibroblasts in SDHD -CBT is more abundant, with significant enrichment in pathways such as FGF, PTN, WNT, PROS, PERIOSTIN, and TGFb. Conclusion: Endothelial cells and fibroblasts in SDHD -CBT are heterogeneous and involved in important cellular interactionprocesses, in which the discovery of FGF,PTN,WNT,PROS,PERIOSTIN and TGFb signals may play an important role in the regulation of microenvironment of SDHD -CBT.<br />Competing Interests: The authors of this article and the planning committee members and staff have no relevant financial relationships with commercial interests to disclose.<br /> (Copyright© by the Editorial Department of Journal of Clinical Otorhinolaryngology Head and Neck Surgery.)
- Subjects :
- Humans
Carotid Body Tumor metabolism
Carotid Body Tumor genetics
Carotid Body Tumor pathology
Signal Transduction
Succinate Dehydrogenase metabolism
Succinate Dehydrogenase genetics
Computational Biology methods
Paraganglioma genetics
Paraganglioma pathology
Paraganglioma metabolism
Cell Communication
Mutation
Cell Adhesion Molecules metabolism
Cell Adhesion Molecules genetics
Endothelial Cells metabolism
Tumor Microenvironment
Fibroblasts metabolism
Subjects
Details
- Language :
- Chinese
- ISSN :
- 2096-7993
- Volume :
- 38
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Lin chuang er bi yan hou tou jing wai ke za zhi = Journal of clinical otorhinolaryngology, head, and neck surgery
- Publication Type :
- Academic Journal
- Accession number :
- 39193734
- Full Text :
- https://doi.org/10.13201/j.issn.2096-7993.2024.09.003