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Polystyrene nanoplastics significantly facilitate largemouth bass ranavirus infection of host cells.
- Source :
-
Journal of hazardous materials [J Hazard Mater] 2024 Oct 05; Vol. 478, pp. 135597. Date of Electronic Publication: 2024 Aug 23. - Publication Year :
- 2024
-
Abstract
- Novel pollutants nanoplastics (NPs) are widely distributed in aquatic environments and may pose a health threat to aquatic organisms. Notably, the contribution of NPs to the occurrence of viral diseases in aquatic animals remains largely uncertain. In this study, the effects of polystyrene nanoplastics (PS-NPs) on Largemouth bass ranavirus (LMBV)-infected MsF cells were investigated. MsF cells took up PS-NPs in a time- and dose-dependent manner and significantly affect cell viability at an exposure concentration of 500 μg/mL. Western blot and qPCR assays indicated that exposure to PS-NPs accelerated LMBV replication in MsF cells. PS-NPs act synergistically with LMBV to disrupt the cellular antioxidant system, as evidenced by increased ROS production and decreased mRNA levels of antioxidant-associated genes. Furthermore, PS-NPs was found to exacerbate LMBV-induced inflammatory responses, as demonstrated by disturbed expression of inflammation-related factors. In addition, our results suggest that PS-NPs reduce IFN production by inhibiting the expression of molecules related to the cGAS-STING signaling pathway, thereby promoting viral replication. Collectively, our findings suggest the potential threat of NPs to infectious diseases caused by freshwater fish viruses and provide new insights for fish disease prevention and control.<br />Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br /> (Copyright © 2024 Elsevier B.V. All rights reserved.)
- Subjects :
- Animals
Reactive Oxygen Species metabolism
Cell Survival drug effects
Water Pollutants, Chemical toxicity
Nanoparticles toxicity
Nanoparticles chemistry
Cell Line
Ranavirus drug effects
Bass virology
Polystyrenes toxicity
Polystyrenes chemistry
DNA Virus Infections virology
DNA Virus Infections veterinary
Virus Replication drug effects
Fish Diseases virology
Subjects
Details
- Language :
- English
- ISSN :
- 1873-3336
- Volume :
- 478
- Database :
- MEDLINE
- Journal :
- Journal of hazardous materials
- Publication Type :
- Academic Journal
- Accession number :
- 39182289
- Full Text :
- https://doi.org/10.1016/j.jhazmat.2024.135597