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Analgesic targets identified in mouse sensory neuron somata and terminal pain translatomes.
- Source :
-
Cell reports [Cell Rep] 2024 Aug 27; Vol. 43 (8), pp. 114614. Date of Electronic Publication: 2024 Aug 19. - Publication Year :
- 2024
-
Abstract
- The relationship between transcription and protein expression is complex. We identified polysome-associated RNA transcripts in the somata and central terminals of mouse sensory neurons in control, painful (plus nerve growth factor), and pain-free conditions (Nav1.7-null mice). The majority (98%) of translated transcripts are shared between male and female mice in both the somata and terminals. Some transcripts are highly enriched in the somata or terminals. Changes in the translatome in painful and pain-free conditions include novel and known regulators of pain pathways. Antisense knockdown of selected somatic and terminal polysome-associated transcripts that correlate with pain states diminished pain behavior. Terminal-enriched transcripts included those encoding synaptic proteins (e.g., synaptotagmin), non-coding RNAs, transcription factors (e.g., Znf431), proteins associated with transsynaptic trafficking (HoxC9), GABA-generating enzymes (Gad1 and Gad2), and neuropeptides (Penk). Thus, central terminal translation may well be a significant regulatory locus for peripheral input from sensory neurons.<br />Competing Interests: Declaration of interests The authors declare no competing interests.<br /> (Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Mice
Male
Female
Protein Biosynthesis
NAV1.7 Voltage-Gated Sodium Channel metabolism
NAV1.7 Voltage-Gated Sodium Channel genetics
Glutamate Decarboxylase metabolism
Glutamate Decarboxylase genetics
Polyribosomes metabolism
Mice, Inbred C57BL
Ganglia, Spinal metabolism
Sensory Receptor Cells metabolism
Pain metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2211-1247
- Volume :
- 43
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Cell reports
- Publication Type :
- Academic Journal
- Accession number :
- 39163201
- Full Text :
- https://doi.org/10.1016/j.celrep.2024.114614