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Screening of differentially expressed RNAs and identifying a ceRNA axis during cadmium-induced oxidative damage in pancreatic β cells.
- Source :
-
Scientific reports [Sci Rep] 2024 Aug 16; Vol. 14 (1), pp. 18962. Date of Electronic Publication: 2024 Aug 16. - Publication Year :
- 2024
-
Abstract
- Cadmium, a common metal pollutant, has been demonstrated to induce type 2 diabetes by disrupting pancreatic β cells function. In this study, transcriptome microarray was utilized to identify differential gene expression in oxidative damage to pancreatic β cells following cadmium exposure. The results indicated that a series of mRNAs, LncRNAs, and miRNAs were altered. Of the differentially expressed miRNAs, miR-29a-3p exhibited the most pronounced alteration, with an 11.62-fold increase relative to the control group. Following this, the target gene of miR-29a-3p was identified as Col3a1 through three databases (miRDB, miRTarbase and Tarbase), which demonstrated a decrease across the transcriptome microarray. The upstream target gene of miR-29a-3p was identified as NONMMUT036805, with decreased expression observed in the microarray. Finally, the expression trend of NONMMUT036805/miR-29a-3p/Col3a1 was reversed following NAC pretreatment. This was accompanied by a reduction in oxidative damage indicators, MDA/ROS/GSH-Px appeared to be negatively affected to varying degrees. In conclusion, this study has demonstrated that multiple RNAs are altered during cadmium exposure-induced oxidative damage in pancreatic β cells. The NONMMUT036805/miR-29a-3p/Col3a1 axis has been shown to be involved in this process, which provides a foundation for the identification of potential targets for cadmium toxicity intervention.<br /> (© 2024. The Author(s).)
- Subjects :
- Animals
Mice
Cell Line
Gene Expression Profiling
Gene Expression Regulation drug effects
Gene Regulatory Networks drug effects
RNA, Long Noncoding genetics
RNA, Long Noncoding metabolism
RNA, Messenger genetics
RNA, Messenger metabolism
Transcriptome
Cadmium toxicity
Insulin-Secreting Cells metabolism
Insulin-Secreting Cells drug effects
MicroRNAs genetics
MicroRNAs metabolism
Oxidative Stress drug effects
Oxidative Stress genetics
RNA, Competitive Endogenous genetics
RNA, Competitive Endogenous metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 14
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 39152192
- Full Text :
- https://doi.org/10.1038/s41598-024-69937-9