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Ex vivo delivery of dsRNA targeting ryanodine receptors for control of Tuta absoluta.
- Source :
-
Pest management science [Pest Manag Sci] 2024 Aug 16. Date of Electronic Publication: 2024 Aug 16. - Publication Year :
- 2024
- Publisher :
- Ahead of Print
-
Abstract
- Background: RNA interference (RNAi) is an endogenous eukaryote viral defence mechanism representing a unique form of post-transcriptional gene silencing. Owing to its high specificity, this technology is being developed for use in dsRNA-based biopesticides for control of pest insects. Whilst many lepidopteran species are recalcitrant to RNAi, Tuta absoluta, a polyphagous insect responsible for extensive crop damage, is sensitive. Ryanodine receptors (RyRs) are intracellular calcium channels regulating calcium ion (Ca <superscript>2+</superscript> ) release. The chemical pesticide class of diamides functions agonistically against lepidopteran RyR, resulting in uncontrolled Ca <superscript>2+</superscript> release, feeding cessation and death. Resistance to diamides has emerged in T. absoluta, derived from RyR point mutations.<br />Results: RNAi was used to target RyR transcripts of T. absoluta. Data presented here demonstrate the systemic use of exogenous T. absoluta RyR-specific (TaRy) dsRNA in tomato plants (Solanum lycopersicum) to significantly downregulate expression of the target gene, resulting in significant insect mortality and reduced leaf damage. Using a leaflet delivery system, daily dosing of 3 μg TaRy dsRNA for 72 h resulted in 50% downregulation of the target gene and 50% reduction in tomato leaf damage. Corrected larval mortality and adult emergence were reduced by 38% and 33%, respectively. TaRy dsRNA demonstrated stability in tomato leaves ≤72 h after dosing.<br />Conclusions: This work identifies TaRy as a promising target for RNAi control of this widespread crop pest. © 2024 The Author(s). Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.<br /> (© 2024 The Author(s). Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.)
Details
- Language :
- English
- ISSN :
- 1526-4998
- Database :
- MEDLINE
- Journal :
- Pest management science
- Publication Type :
- Academic Journal
- Accession number :
- 39148493
- Full Text :
- https://doi.org/10.1002/ps.8368