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Contribution of Surface Adhesins of Lacticaseibacillus paracasei S -NB to Its Intestinal Adhesion and Colonization.

Contribution of Surface Adhesins of Lacticaseibacillus paracasei S -NB to Its Intestinal Adhesion and Colonization.

Authors :
Xiao L
Zhao X
Lin L
Mahsa GC
Ma K
Zhang C
Rui X
Li W
Source :
Journal of agricultural and food chemistry [J Agric Food Chem] 2024 Aug 28; Vol. 72 (34), pp. 18986-19002. Date of Electronic Publication: 2024 Aug 14.
Publication Year :
2024

Abstract

The intestinal retention and persistence of lactic acid bacteria (LAB) are strain-specific and affected by the bacterial surface components. However, the contribution of surface adhesins of LAB to intestinal adhesion and colonization remains unclear. In the present study, seven gene knockout mutants (genes related to surface adhesin synthesis) of Lacticaseibacillus paracasei S -NB were derived based on the Cre- lox -based recombination system. Results showed that the capsule layer appeared thinner in the cell wall of S -NBΔ7576, S -NBΔ dlt , and S -NBΔ srtA mutants when compared with the wild-type (WT) S -NB. The effects of S-NB _7576 ( wzd and wze genes, responsible for capsular polysaccharide synthesis) and S-NB _ srtA (sortase A) mutation on the hydrophobicity, surface charge, and adhesion ability seem to vary strongly among seven mutant strains. In vivo colonization experiments showed a decrease in the colonization numbers of S -NBΔ7576 and S -NBΔ srtA in both the ileal and colon lumen from 2 to 8 days when compared with those of the WT S -NB. In conclusion, the synthesis of capsular polysaccharides and the transport of surface proteins are closely related to the adhesion ability and intestinal colonization of L. paracasei S -NB.

Details

Language :
English
ISSN :
1520-5118
Volume :
72
Issue :
34
Database :
MEDLINE
Journal :
Journal of agricultural and food chemistry
Publication Type :
Academic Journal
Accession number :
39140151
Full Text :
https://doi.org/10.1021/acs.jafc.4c04256