Ex Vivo Live Imaging in Brain Slices for Analysis of Neurite Formation in Combination with In Utero Electroporation.

Authors :: Liu X
Toyo-Oka K
Source :: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2024; Vol. 2831, pp. 199-208.
Publication Year :: 2024
Abstract: The use of time-lapse live imaging enables us to track the dynamic changes in neurites during their formation. Ex vivo live imaging with acute brain slices provides a more physiological environment than cultured cells. To accomplish this, a certain method of labeling is necessary to visualize and identify neurite morphology. To understand the dynamics of neurite structure at early stages of neurite formation, we describe in this chapter ex vivo live imaging using a confocal microscope at P0 in combination with in utero electroporation (IUE).<br /> (© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)

Subjects :: Animals
Mice
Female
Microscopy, Confocal methods
Time-Lapse Imaging methods
Pregnancy
Neurogenesis
Electroporation methods
Neurites metabolism
Brain cytology
Brain embryology
Brain diagnostic imaging

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