Back to Search
Start Over
Intraocular fatty acids induce reinforcement of barrier functions on the outer blood-retinal barrier.
- Source :
-
Prostaglandins, leukotrienes, and essential fatty acids [Prostaglandins Leukot Essent Fatty Acids] 2024 Mar; Vol. 202, pp. 102637. Date of Electronic Publication: 2024 Aug 08. - Publication Year :
- 2024
-
Abstract
- The aim of the present study was to elucidate unknown effects of intraocular fatty acids (ioFAs) including palmitic acid (C16:0), stearic acid (C18:0), oleic acid (C18:1), linoleic acid (C18:2), arachidonic acid (C20:4), eicosapentaenoic acid (EPA, C20:5) and docosahexaenoic acid (DHA, C22:6) on the outer blood-retinal barrier (oBRB). For this purpose, human retinal pigment epithelium cell line ARPE19 was subjected to analyses for evaluating the following biological phenotypes: (1) cell viability, (2) cellular metabolic functions, (3) barrier functions by trans-epithelial electrical resistance (TEER), and (4) expression of tight junction (TJ) molecules. In the presence of 100 nM ioFAs, no significant effects on cell viability of ARPE19 cells was observed. While treatment with EPA or DHA tended to reduce non-mitochondrial oxygen consumption, most indices in mitochondrial functions were not markedly affected by treatment with ioFAs in ARPE19 cells. On the other hand, ioFAs except for palmitic acid and stearic acid significantly increased basal extracellular acidification rates, suggesting activated glycolysis or increased lactate production. Interestingly, TEER values of planar ARPE19 monolayer were significantly increased by treatment any ioFAs. Consistently, gene expression levels of TJ proteins were increased by treatment with ioFAs. Collectively, the findings presented herein suggest that ioFAs may contribute to reinforcement of barrier functions of the oBRB albeit there are some differences in biological effects depending on the type of ioFAs.<br /> (Copyright © 2024 Elsevier Ltd. All rights reserved.)
- Subjects :
- Humans
Cell Line
Fatty Acids metabolism
Fatty Acids pharmacology
Cell Survival drug effects
Palmitic Acid pharmacology
Docosahexaenoic Acids pharmacology
Stearic Acids pharmacology
Linoleic Acid pharmacology
Eicosapentaenoic Acid pharmacology
Oleic Acid pharmacology
Tight Junctions metabolism
Tight Junctions drug effects
Arachidonic Acid pharmacology
Arachidonic Acid metabolism
Blood-Retinal Barrier metabolism
Blood-Retinal Barrier drug effects
Retinal Pigment Epithelium metabolism
Retinal Pigment Epithelium drug effects
Retinal Pigment Epithelium cytology
Subjects
Details
- Language :
- English
- ISSN :
- 1532-2823
- Volume :
- 202
- Database :
- MEDLINE
- Journal :
- Prostaglandins, leukotrienes, and essential fatty acids
- Publication Type :
- Academic Journal
- Accession number :
- 39128394
- Full Text :
- https://doi.org/10.1016/j.plefa.2024.102637