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CC-seq: Nucleotide-Resolution Mapping of Spo11 DNA Double-Strand Breaks in S. cerevisiae Cells.
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2024; Vol. 2818, pp. 3-22. - Publication Year :
- 2024
-
Abstract
- During meiosis, Spo11 generates DNA double-strand breaks to induce recombination, becoming covalently attached to the 5' ends on both sides of the break during this process. Such Spo11 "covalent complexes" are transient in wild-type cells, but accumulate in nuclease mutants unable to initiate repair. The CC-seq method presented here details how to map the location of these Spo11 complexes genome-wide with strand-specific nucleotide-resolution accuracy in synchronized Saccharomyces cerevisiae meiotic cells.<br /> (© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
- Subjects :
- Saccharomyces cerevisiae Proteins genetics
Saccharomyces cerevisiae Proteins metabolism
DNA, Fungal genetics
DNA, Fungal metabolism
Sequence Analysis, DNA methods
DNA Repair
Saccharomyces cerevisiae genetics
DNA Breaks, Double-Stranded
Endodeoxyribonucleases metabolism
Endodeoxyribonucleases genetics
Meiosis genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1940-6029
- Volume :
- 2818
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 39126464
- Full Text :
- https://doi.org/10.1007/978-1-0716-3906-1_1