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Effect of Torilis japonica Fruit Extract for Endothelium-Independent Vasorelaxation and Blood Pressure Lowering in Rats.
- Source :
-
International journal of molecular sciences [Int J Mol Sci] 2024 Jul 25; Vol. 25 (15). Date of Electronic Publication: 2024 Jul 25. - Publication Year :
- 2024
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Abstract
- Torilis japonica (TJ) fruit, is a herb that is traditionally used for erectile dysfunction (ED). Given the shared mechanisms of ED and hypertension through vascular smooth muscle, we hypothesized that TJ would be effective in vasodilation and blood pressure reduction. This study confirmed the authenticity of TJ samples via DNA barcoding and quantified the main active compound, torilin, using HPLC. TJ was extracted with distilled water (TJW) and 50% ethanol (TJE), yielding torilin contents of 0.35 ± 0.01% and 2.84 ± 0.02%, respectively. Ex vivo tests on thoracic aortic rings from Sprague-Dawley rats showed that TJE (3-300 µg/mL) induced endothelium-independent, concentration-dependent vasodilation, unlike TJW. Torilin caused concentration-dependent relaxation with an EC <subscript>50</subscript> of 210 ± 1.07 µM. TJE's effects were blocked by a voltage-dependent K <superscript>+</superscript> channel blocker and alleviated contractions induced by CaCl <subscript>2</subscript> and angiotensin II. TJE inhibited vascular contraction induced by phenylephrine or KCl via extracellular CaCl <subscript>2</subscript> and enhanced inhibition with nifedipine, indicating involvement of voltage-dependent and receptor-operated Ca <superscript>2+</superscript> channels. Oral administration of TJE (1000 mg/kg) significantly reduced blood pressure in spontaneously hypertensive rats. These findings suggest TJ extract's potential for hypertension treatment through vasorelaxant mechanisms, though further research is needed to confirm its efficacy and safety.
- Subjects :
- Animals
Rats
Male
Antihypertensive Agents pharmacology
Vasodilator Agents pharmacology
Aorta, Thoracic drug effects
Aorta, Thoracic metabolism
Rats, Inbred SHR
Muscle, Smooth, Vascular drug effects
Muscle, Smooth, Vascular metabolism
Hypertension drug therapy
Hypertension metabolism
Hypertension physiopathology
Vasodilation drug effects
Plant Extracts pharmacology
Blood Pressure drug effects
Fruit chemistry
Rats, Sprague-Dawley
Endothelium, Vascular drug effects
Endothelium, Vascular metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1422-0067
- Volume :
- 25
- Issue :
- 15
- Database :
- MEDLINE
- Journal :
- International journal of molecular sciences
- Publication Type :
- Academic Journal
- Accession number :
- 39125672
- Full Text :
- https://doi.org/10.3390/ijms25158101