Genomic analysis and metabolic characteristics provide insights into inorganic nitrogen metabolism of novel bacterium Acinetobacter pittii J08.

A novel A. pittii J08 with heterotrophic nitrification and aerobic denitrification (HN-AD) isolated from pond sediments could rapidly degrade inorganic nitrogen (N) and total nitrogen (TN-N) with ammonium (NH ₄ ⁺ -N) preference. N degradation rate of NH ₄ ⁺ -N, nitrite (NO ₂ ^- -N) and nitrate (NO ₃ ^- -N) were 3.9 mgL ^-1 h ^-1 , 3.0 mgL ^-1 h ^-1 and 2.7 mgL ^-1 h ^-1 , respectively. In addition, strain J08 could effectively utilize most of detected low-molecular-weight carbon (LMWC) sources to degrade inorganic N with a wide adaptability to various culture conditions. Whole genome sequencing (WGS) analysis revealed that assembled genome of stain J08 possessed the crucial genes involved in dissimilatory/assimilatory NO ₃ ^- -N reduction and NH ₄ ⁺ -N assimilation. These results indicated that strain J08 could be applied to wastewater treatment in aquaculture.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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