Back to Search
Start Over
Deciphering deep-sea chemosynthetic symbiosis by single-nucleus RNA-sequencing.
- Source :
-
ELife [Elife] 2024 Aug 05; Vol. 12. Date of Electronic Publication: 2024 Aug 05. - Publication Year :
- 2024
-
Abstract
- Bathymodioline mussels dominate deep-sea methane seep and hydrothermal vent habitats and obtain nutrients and energy primarily through chemosynthetic endosymbiotic bacteria in the bacteriocytes of their gill. However, the molecular mechanisms that orchestrate mussel host-symbiont interactions remain unclear. Here, we constructed a comprehensive cell atlas of the gill in the mussel Gigantidas platifrons from the South China Sea methane seeps (1100 m depth) using single-nucleus RNA-sequencing (snRNA-seq) and whole-mount in situ hybridisation. We identified 13 types of cells, including three previously unknown ones, and uncovered unknown tissue heterogeneity. Every cell type has a designated function in supporting the gill's structure and function, creating an optimal environment for chemosynthesis, and effectively acquiring nutrients from the endosymbiotic bacteria. Analysis of snRNA-seq of in situ transplanted mussels clearly showed the shifts in cell state in response to environmental oscillations. Our findings provide insight into the principles of host-symbiont interaction and the bivalves' environmental adaption mechanisms.<br />Competing Interests: HW, KH, HZ, QZ, LC, JL, ZZ, HC, LZ, CL, MW, KC, PQ, CL No competing interests declared<br /> (© 2023, Wang, He, Zhang et al.)
Details
- Language :
- English
- ISSN :
- 2050-084X
- Volume :
- 12
- Database :
- MEDLINE
- Journal :
- ELife
- Publication Type :
- Academic Journal
- Accession number :
- 39102287
- Full Text :
- https://doi.org/10.7554/eLife.88294