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Whole genome sequencing insight into carbapenem-resistant and multidrug-resistant Acinetobacter baumannii harboring chromosome-borne bla OXA-23 .
- Source :
-
Microbiology spectrum [Microbiol Spectr] 2024 Sep 03; Vol. 12 (9), pp. e0050124. Date of Electronic Publication: 2024 Aug 05. - Publication Year :
- 2024
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Abstract
- Carbapenem-resistant Acinetobacter baumannii (CRAB) poses a significant threat to hospitalized patients as effective therapeutic options are scarce. Based on the genomic characteristics of the CRAB strain AB2877 harboring chromosome-borne bla <subscript>OXA-23</subscript> , which was isolated from the bronchoalveolar lavage fluid (BALF) of a patient in a respiratory intensive care unit (RICU), we systematically analyzed antibiotic resistance genes (ARGs) and the genetic context associated with ARGs carried by CRAB strains harboring chromosome-borne bla <subscript>OXA-23</subscript> worldwide. Besides bla <subscript>OXA-23</subscript> , other ARGs were detected on the chromosome of the CRAB strain AB2877 belonging to ST208/1806 (Oxford MLST scheme). Several key genetic contexts associated with the ARGs were identified on the chromosome of the CRAB strain AB2877, including (1) the MDR region associated with bla <subscript>OXA-23</subscript> , tet(B)-tetR(B ), aph(3'')-Ib, and aph(6)-Id (2); the resistance island AbGRI3 harboring armA and mph(E)-msr(E ) (3); the Tn 3 -like composite transposon containing bla <subscript>TEM-1D</subscript> and aph(3')-Ia ; and (4) the structure "IS Aba1-bla <subscript>ADC-25</subscript> ." The first two genetic contexts were most common in ST195/1816, followed by ST208/1806. The last two genetic contexts were found most frequently in ST208/1806, followed by ST195/1816.IMPORTANCEThe bla <subscript>OXA-23</subscript> gene can be carried by plasmid or chromosome, facilitating horizontal genetic transfer and increasing carbapenem resistance in healthcare settings. In this study, we focused on the genomic characteristics of CRAB strains harboring the chromosome-borne bla <subscript>OXA-23</subscript> gene, and the important genetic contexts associated with bla <subscript>OXA-23</subscript> and other ARGs were identified, and their prevalent clones worldwide were determined. Notably, although the predominant clonal CRAB lineages worldwide containing the MDR region associated with bla <subscript>OXA-23</subscript> , tet(B)-tetR(B ), aph(3'')-Ib, and aph (6)-Id was ST195/1816, followed by ST208/1806, the CRAB strain AB2877 in our study belonged to ST208/1806. Our findings contribute to the knowledge regarding the dissemination of CRAB strains and the control of nosocomial infection.<br />Competing Interests: The authors declare no conflict of interest.
- Subjects :
- Humans
Acinetobacter Infections microbiology
Acinetobacter Infections drug therapy
Bacterial Proteins genetics
Bacterial Proteins metabolism
Chromosomes, Bacterial genetics
Genome, Bacterial
Microbial Sensitivity Tests
Multilocus Sequence Typing
Whole Genome Sequencing
Acinetobacter baumannii genetics
Acinetobacter baumannii drug effects
Anti-Bacterial Agents pharmacology
beta-Lactamases genetics
Carbapenems pharmacology
Drug Resistance, Multiple, Bacterial genetics
Subjects
Details
- Language :
- English
- ISSN :
- 2165-0497
- Volume :
- 12
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Microbiology spectrum
- Publication Type :
- Academic Journal
- Accession number :
- 39101706
- Full Text :
- https://doi.org/10.1128/spectrum.00501-24