Bodipy-Based highly sensitive hydrogen sulfide fluorescent probe and its fluorescence imaging in cells and zebrafish.

Hydrogen sulfide (H ₂ S) is a crucial endogenous gasotransmitter that plays a role in various physiological and pathological processes. Therefore, accurate and rapid monitoring of H ₂ S in organisms is highly significant for understanding the underlying pathological mechanisms and facilitating early diagnosis of related diseases. In this study, we developed a novel fluorescent probe, B-CHO-NO ₂ , based on a bodipy fluorophore, which exhibits excellent sensitivity and selectivity towards H ₂ S. The design of the probe exploits the nucleophilicity of H ₂ S by introducing a formyl group as the ortho-participating moiety, significantly enhancing the reaction rate with H ₂ S. In cellular and zebrafish models, the probe B-CHO-NO ₂ successfully achieved fluorescence imaging of endogenous and exogenous H ₂ S. The development of probe B-CHO-NO ₂ provides a powerful tool for biological studies of H ₂ S and diagnosis of related diseases.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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