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Effect of rhamnogalacturonan-I-rich polysaccharides isolated from crabapple hydrolysates on IL-1β-induced inflammation in intestinal epithelial cells.
- Source :
-
International journal of biological macromolecules [Int J Biol Macromol] 2024 Oct; Vol. 277 (Pt 3), pp. 134240. Date of Electronic Publication: 2024 Jul 31. - Publication Year :
- 2024
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Abstract
- This study aimed to investigate the structural characteristics and intracellular mechanisms of polysaccharides (MP-PE-I) purified from a crabapple (Malus prunifolia) enzymatic hydrolysate (MP-PE). Activity-guided fractionation revealed that MP-PE-I was the active moiety and significantly reduced the production and gene expression of pro-inflammatory factors in interleukin (IL)-1β-treated intestinal epithelial cells (Caco-2). Moreover, MP-PE-I downregulated the phosphorylation and nuclear localization of proteins involved in the mitogen-activated protein kinase (MAPK) and nuclear factor-κB (NF-κB) pathways, as evidenced by immunoblotting and immunofluorescence analysis. In antagonistic studies with specific inhibitors of the MAPK and NF-κB pathways, IL-6 inhibition was significantly regulated by p38; IL-8 by IκBα, JNK, and p38; and monocyte chemoattractant protein-1 (MCP-1) by JNK, p38, and ERK. Additionally, MP-PE-I significantly decreased the mRNA and protein expression of IL-1 receptor type 1. Chemical and structural characteristic analyses showed that MP-PE-I is a polysaccharide rich in rhamnogalacturonan (RG)-I and plays a crucial role in intestinal immunomodulation. To our knowledge, this is the first study to demonstrate the intestinal immunomodulatory activity, intracellular mechanisms, and structural characteristics of RG-I-rich polysaccharides isolated from crabapples.<br />Competing Interests: Declaration of competing interest The authors declare that they have no competing interests.<br /> (Copyright © 2024 Elsevier B.V. All rights reserved.)
- Subjects :
- Humans
Inflammation chemically induced
Inflammation drug therapy
Inflammation metabolism
Caco-2 Cells
Epithelial Cells drug effects
Epithelial Cells metabolism
Intestinal Mucosa drug effects
Intestinal Mucosa metabolism
NF-kappa B metabolism
Hydrolysis
Mitogen-Activated Protein Kinases metabolism
Pectins pharmacology
Pectins chemistry
Pectins isolation & purification
Interleukin-1beta metabolism
Interleukin-1beta genetics
Polysaccharides pharmacology
Polysaccharides chemistry
Polysaccharides isolation & purification
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0003
- Volume :
- 277
- Issue :
- Pt 3
- Database :
- MEDLINE
- Journal :
- International journal of biological macromolecules
- Publication Type :
- Academic Journal
- Accession number :
- 39094865
- Full Text :
- https://doi.org/10.1016/j.ijbiomac.2024.134240