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DNA tetrahedral nanocages as a promising nanocarrier for dopamine delivery in neurological disorders.
- Source :
-
Nanoscale [Nanoscale] 2024 Aug 15; Vol. 16 (32), pp. 15158-15169. Date of Electronic Publication: 2024 Aug 15. - Publication Year :
- 2024
-
Abstract
- Dopamine is a neurotransmitter in the central nervous system that is essential for many bodily and mental processes, and a lack of it can cause Parkinson's disease. DNA tetrahedral (TD) nanocages are promising in bio-nanotechnology, especially as a nanocarrier. TD is highly programmable, biocompatible, and capable of cell differentiation and proliferation. It also has tissue and blood-brain barrier permeability, making it a powerful tool that could overcome potential barriers in treating neurological disorders. In this study, we used DNA TD as a carrier for dopamine to cells and zebrafish embryos. We investigated the mechanism of complexation between TD and dopamine hydrochloride using gel electrophoresis, fluorescence and circular dichroism (CD) spectroscopy, atomic force microscopy (AFM), and molecular dynamic (MD) simulation tools. Further, we demonstrate that these dopamine-loaded DNA TD nanostructures enhanced cellular uptake and differentiation ability in SH-SY5Y neuroblastoma cells. Furthermore, we extended the study to zebrafish embryos as a model organism to examine survival and uptake. The research provides valuable insights into the complexation mechanism and cellular uptake of dopamine-loaded DNA tetrahedral nanostructures, paving the way for further advancements in nanomedicine for Parkinson's disease and other neurological disorders.
- Subjects :
- Animals
Humans
Cell Line, Tumor
Nanostructures chemistry
Parkinson Disease drug therapy
Parkinson Disease metabolism
Molecular Dynamics Simulation
Nervous System Diseases drug therapy
Nervous System Diseases metabolism
Cell Differentiation drug effects
Blood-Brain Barrier metabolism
Zebrafish
Dopamine chemistry
Dopamine metabolism
Dopamine pharmacology
DNA chemistry
DNA metabolism
Drug Carriers chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 2040-3372
- Volume :
- 16
- Issue :
- 32
- Database :
- MEDLINE
- Journal :
- Nanoscale
- Publication Type :
- Academic Journal
- Accession number :
- 39091152
- Full Text :
- https://doi.org/10.1039/d4nr00612g