Flow Cytometry-based Immune Phenotyping of T and B Lymphocytes in the Evaluation of Immunodeficiency and Immune Dysregulation.

There are approximately 500 congenital disorders that impair immune cell development and/or function. Patients with these disorders may present with a wide range of symptoms, including increased susceptibility to infection, autoimmunity, autoinflammation, lymphoproliferation, and/or atopy. Flow cytometry-based immune phenotyping of T and B lymphocytes plays an essential role in the evaluation of patients with these presentations. In this review, we describe the clinical utility of flow cytometry as part of a comprehensive evaluation of immune function and how this testing may be used as a diagnostic tool to identify underlying aberrant immune pathways, monitor disease activity, and assess infection risk.
Competing Interests: Disclosure A. A. Nguyen is a staff physician at Boston Children’s Hospital and Instructor of Pediatrics at Harvard Medical School. C. D. Platt is a staff physician and Medical Director of Flow Cytometry at Boston Children’s Hospital, and Assistant Professor of Pediatrics at Harvard Medical School. The spouse of C. D. Platt is employed by Quest Diagnostics. A. A. Nguyen has nothing to disclose.
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