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Critical factors for precise and efficient RNA cleavage by RNase Y in Staphylococcus aureus.
- Source :
-
PLoS genetics [PLoS Genet] 2024 Aug 01; Vol. 20 (8), pp. e1011349. Date of Electronic Publication: 2024 Aug 01 (Print Publication: 2024). - Publication Year :
- 2024
-
Abstract
- Cellular processes require precise and specific gene regulation, in which continuous mRNA degradation is a major element. The mRNA degradation mechanisms should be able to degrade a wide range of different RNA substrates with high efficiency, but should at the same time be limited, to avoid killing the cell by elimination of all cellular RNA. RNase Y is a major endoribonuclease found in most Firmicutes, including Bacillus subtilis and Staphylococcus aureus. However, the molecular interactions that direct RNase Y to cleave the correct RNA molecules at the correct position remain unknown. In this work we have identified transcripts that are homologs in S. aureus and B. subtilis, and are RNase Y targets in both bacteria. Two such transcript pairs were used as models to show a functional overlap between the S. aureus and the B. subtilis RNase Y, which highlighted the importance of the nucleotide sequence of the RNA molecule itself in the RNase Y targeting process. Cleavage efficiency is driven by the primary nucleotide sequence immediately downstream of the cleavage site and base-pairing in a secondary structure a few nucleotides downstream. Cleavage positioning is roughly localised by the downstream secondary structure and fine-tuned by the nucleotide immediately upstream of the cleavage. The identified elements were sufficient for RNase Y-dependent cleavage, since the sequence elements from one of the model transcripts were able to convert an exogenous non-target transcript into a target for RNase Y.<br />Competing Interests: The authors have declared that no competing interests exist.<br /> (Copyright: © 2024 Le Scornet et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
- Subjects :
- RNA, Messenger genetics
RNA, Messenger metabolism
Bacterial Proteins metabolism
Bacterial Proteins genetics
Endoribonucleases metabolism
Endoribonucleases genetics
Nucleic Acid Conformation
Base Sequence
Staphylococcus aureus genetics
Staphylococcus aureus enzymology
Bacillus subtilis genetics
Bacillus subtilis enzymology
Bacillus subtilis metabolism
RNA, Bacterial metabolism
RNA, Bacterial genetics
RNA Stability genetics
Gene Expression Regulation, Bacterial
RNA Cleavage
Subjects
Details
- Language :
- English
- ISSN :
- 1553-7404
- Volume :
- 20
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- PLoS genetics
- Publication Type :
- Academic Journal
- Accession number :
- 39088561
- Full Text :
- https://doi.org/10.1371/journal.pgen.1011349