Back to Search Start Over

Discovery of Novel α,β-Unsaturated Amide Derivatives as Candidate Antifungals to Overcome Fungal Resistance.

Authors :
Yan Z
Li Q
Li X
Wang H
Zhao D
Yu H
Guo M
Wang Y
Wang X
Xu H
Mou Y
Hou Z
Guo C
Source :
Journal of medicinal chemistry [J Med Chem] 2024 Aug 08; Vol. 67 (15), pp. 12601-12617. Date of Electronic Publication: 2024 Jul 30.
Publication Year :
2024

Abstract

In our previous study, coumarin-containing CYP51 inhibitor A32 demonstrated potent antiresistance activity. However, compound A32 demonstrated unsatisfied metabolic stability, necessitating modifications to overcome these limitations. In this study, α,β-unsaturated amides were used to replace the unstable coumarin ring, which increased metabolic stability by four times while maintaining antifungal activity, including activity against resistant strains. Subsequently, the sterol composition analysis and morphological observation experiments indicated that the target of these novel compounds is lanosterol 14α-demethylase (CYP51). Meanwhile, biofilm growth was inhibited and resistance genes ( ERG11 , CDR1 , CDR2 , and MDR1 ) expression was downregulated to find out how the antiresistance works. Importantly, compound C07 demonstrated the capacity to stimulate reactive oxygen species, thus displaying potent fungicidal activity. Moreover, C07 exhibited encouraging effectiveness in vivo following intraperitoneal administration. Additionally, the most potent compound C07 showed satisfactory pharmacokinetic properties and low toxicity. These α,β-unsaturated amide derivatives, particularly C07 , are potential candidates for treating azole-resistant candidiasis.

Details

Language :
English
ISSN :
1520-4804
Volume :
67
Issue :
15
Database :
MEDLINE
Journal :
Journal of medicinal chemistry
Publication Type :
Academic Journal
Accession number :
39077891
Full Text :
https://doi.org/10.1021/acs.jmedchem.4c00593