Downhill excitation energy flow in reaction centers of purple bacteria Rhodospirillum rubrum G9.

Using femtosecond differential spectroscopy, excitation energy transfer in reaction centers (RCs) of the carotenoidless strain of purple bacteria Rhodospirillum rubrum G9 was studied at room temperature. Excitation and probing of the Q _y , Q _x and Soret absorption bands of the RCs were carried out by pulses with duration of 25-30 fs. Modeling of ΔA (light - dark) kinetics made it possible to estimate the characteristic time of various stages of excitation energy transformation. It is shown that the dynamics of the downhill energy flow in the RCs is determined both by the internal energy conversion Soret→ Q _x  → Q _y in each cofactor and by the energy transfer H* → B* → P* (H - bacteriopheophytin, B - bacteriochlorophyll a, P - bacteriochlorophyll a dimer) between cofactors. The transfer of energy between the upper excited levels (Soret and Q _x ) of the cofactors accelerates its arrival to the lower exciton level of the P, from where charge separation begins. It turned out that all conversion and energy transfer processes occur within 40-160 fs: the conversion Soret → Q _x occurs in 40-50 fs, the conversion Q _x  → Q _y occurs in 100-140 fs, the transfer H* → B* has a time constant of 80-120 fs, and the transfer B* → P* has a time constant of 130-160 fs. The rate of energy transfer between the upper excited levels is close to the rate of transfer between Q _y levels.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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