Construction of an effective near-infrared fluorescence "turn-on" probe for hydrogen sulfide detection and imaging in living inflammatory cell and zebrafish models.

Hydrogen sulfide (H ₂ S) can act as a gaseous signaling mediator closely associated with inflammation development. In this work, we designed a fluorescence turn-on near-infrared (NIR) fluorescent probe CIT-H ₂ S based on Intermolecular Charge Transfer (ICT) for the detection of H ₂ S in living inflammatory cells and zebrafish. On this basis, a dicyanoisophorone fluorophore was chosen as the fluorescence signal reporting group of CIT-H ₂ S, and an azide group was constructed as the recognition group of H ₂ S. CIT-H ₂ S is characterized by high selectivity and sensitivity for H ₂ S over other interference species. The fluorescence intensity at 661 nm showed good linearity in the range of H ₂ S concentration from 0 to 10 μM, with an excellent limit of detection (LOD) as low as 81.52 nM. Impressively, CIT-H ₂ S has been visualized for detecting H ₂ S in drug-induced inflammatory cell and zebrafish models, thus indicating that CIT-H ₂ S is a robust tool with the ability to study the occurrence and development of hydrogen sulfide and inflammation.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)