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Approaches to scarless burn wound healing: application of 3D printed skin substitutes with dual properties of anti-infection and balancing wound hydration levels.
- Source :
-
EBioMedicine [EBioMedicine] 2024 Aug; Vol. 106, pp. 105258. Date of Electronic Publication: 2024 Jul 27. - Publication Year :
- 2024
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Abstract
- Background: Severe burn wounds face two primary challenges: dysregulated cellular impairment functions following infection and an unbalanced wound hydration microenvironment leading to excessive inflammation and collagen deposition. These results in hypertrophic scar contraction, causing significant deformity and disability in survivors.<br />Methods: A three-dimensional (3D) printed double-layer hydrogel (DLH) was designed and fabricated to address the problem of scar formation after burn injury. DLH was developed using methacrylated silk fibroin (SFMA) and gelatin methacryloyl (GelMA) for the upper layer, and GelMA and hyaluronic acid methacryloyl (HAMA) for the lower layer. To combat infection, copper-epigallocatechin gallate (Cu-EGCG) was incorporated into the lower layer bioink, collectively referred to as DLS. To balance wound hydration levels, HaCaT cells were additionally encapsulated in the upper layer, designed as DLS/c.<br />Findings: DLH demonstrated suitable porosity, appropriate mechanical properties, and excellent biocompatibility. DLS exhibited potent antimicrobial properties, exerted anti-inflammatory effects by regulating macrophage polarisation, and may enhance angiogenesis through the HIF-1α/VEGF pathway. In the DLS/c group, animal studies showed significant improvements in epidermal formation, barrier function, and epidermal hydration, accompanied by reduced inflammation. In addition, Masson's trichrome and Sirius red staining revealed that the structure and ratio of dermal collagen in DLS/c resembled that of normal skin, indicating considerable potential for scarless wound healing.<br />Interpretation: This biomimetic matrix shows promise in addressing the challenges of burn wounds and aiming for scarless repair, with benefits such as anti-infection, epidermal hydration, biological induction, and optimised topological properties.<br />Funding: Shown in Acknowledgements.<br />Competing Interests: Declaration of interests The authors declare no competing interests.<br /> (Copyright © 2024 The Author(s). Published by Elsevier B.V. All rights reserved.)
- Subjects :
- Humans
Animals
Hydrogels chemistry
Mice
Anti-Infective Agents pharmacology
Gelatin chemistry
Cicatrix pathology
Cicatrix metabolism
Cicatrix drug therapy
Cell Line
Fibroins chemistry
Rats
Male
Disease Models, Animal
Burns drug therapy
Burns metabolism
Burns pathology
Wound Healing drug effects
Printing, Three-Dimensional
Skin, Artificial
Subjects
Details
- Language :
- English
- ISSN :
- 2352-3964
- Volume :
- 106
- Database :
- MEDLINE
- Journal :
- EBioMedicine
- Publication Type :
- Academic Journal
- Accession number :
- 39068733
- Full Text :
- https://doi.org/10.1016/j.ebiom.2024.105258