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Identification of Wheat Glutamate Synthetase Gene Family and Expression Analysis under Nitrogen Stress.
- Source :
-
Genes [Genes (Basel)] 2024 Jun 22; Vol. 15 (7). Date of Electronic Publication: 2024 Jun 22. - Publication Year :
- 2024
-
Abstract
- Nitrogen (N), as the main component of biological macromolecules, maintains the basic process of plant growth and development. GOGAT, as a key enzyme in the N assimilation process, catalyzes α-ketoglutaric acid and glutamine to form glutamate. In this study, six GOGAT genes in wheat ( Triticum aestivum L.) were identified and classified into two subfamilies, Fd-GOGAT ( TaGOGAT2s ) and NADH-GOGAT ( TaGOGAT3s ), according to the type of electron donor. Subcellular localization prediction showed that TaGOGAT3-D was localized in mitochondria and that the other five TaGOGATs were localized in chloroplasts. Via the analysis of promoter elements, many binding sites related to growth and development, hormone regulation and plant stress resistance regulations were found on the TaGOGAT promoters. The tissue-specificity expression analysis showed that TaGOGAT2s were mainly expressed in wheat leaves and flag leaves, while TaGOGAT3s were highly expressed in roots and leaves. The expression level of TaGOGATs and the enzyme activity of TaGOGAT3s in the leaves and roots of wheat seedlings were influenced by the treatment of N deficiency. This study conducted a systematic analysis of wheat GOGAT genes, providing a theoretical basis not only for the functional analysis of TaGOGATs , but also for the study of wheat nitrogen use efficiency (NUE).
- Subjects :
- Glutamate Synthase genetics
Glutamate Synthase metabolism
Multigene Family
Promoter Regions, Genetic
Plant Roots genetics
Plant Roots metabolism
Plant Roots growth & development
Seedlings genetics
Seedlings growth & development
Seedlings metabolism
Plant Leaves genetics
Plant Leaves metabolism
Phylogeny
Triticum genetics
Triticum metabolism
Nitrogen metabolism
Gene Expression Regulation, Plant
Stress, Physiological genetics
Plant Proteins genetics
Plant Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2073-4425
- Volume :
- 15
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Genes
- Publication Type :
- Academic Journal
- Accession number :
- 39062606
- Full Text :
- https://doi.org/10.3390/genes15070827