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Label-Free Detection of DNA Base Mutation and Hybridized DNA by an Electrostatically Modified 3D Plasmonic Array.
- Source :
-
ACS sensors [ACS Sens] 2024 Aug 23; Vol. 9 (8), pp. 4119-4126. Date of Electronic Publication: 2024 Jul 23. - Publication Year :
- 2024
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Abstract
- Surface-enhanced Raman scattering (SERS) represents a promising avenue for DNA detection as it offers intrinsic chemical insights with high sensitivity compared to conventional methods. However, label-free and quantitative detection of unmodified DNA by SERS remains a major challenge in DNA analysis. To overcome this challenge, we propose a positively charged plasmonic nanosurface for DNA capture and quantitative analysis. Highly sensitive and uniform SERS enhancement was realized by a three-dimensional plasmonic array supporting well-designed hybrid plasmonic modes. Subsequently, the plasmonic array was modified with an electrostatically functionalized PDDA (poly(diene-dimethylammonium-chloride)) self-assembled monolayer in a single step. The effectiveness of the resulting PDDA-SERS substrate was demonstrated by the label-free and quantitative detection of base content and base mutation in hybridized DNA. The PDDA-SERS substrate provides a robust platform for SERS analysis not only of DNA but also of other electronegative analytes.
Details
- Language :
- English
- ISSN :
- 2379-3694
- Volume :
- 9
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- ACS sensors
- Publication Type :
- Academic Journal
- Accession number :
- 39039996
- Full Text :
- https://doi.org/10.1021/acssensors.4c01060